您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway

文献类型： 外文期刊

作者： Chen, Yuan 1 ; Xia, Jing 1 ; Su, Zhenhe 1 ; Xu, Gaoge 1 ; Gomelsky, Mark 3 ; Qian, Guoliang 1 ; Liu, Fengquan 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Jiangsu, Peoples R China

3.Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

关键词： antibiotics;HSAF;Lysobacter;PilR;type IV pili

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lysobacter enzymogenes is a ubiquitous soil gammaproteobacterium that produces a broad-spectrum antifungal antibiotic, known as heat-stable antifungal factor (HSAF). To increase HSAF production for use against fungal crop diseases, it is important to understand how HSAF synthesis is regulated. To gain insights into transcriptional regulation of the HSAF synthesis gene cluster, we generated a library with deletion mutations in the genes predicted to encode response regulators of the two-component signaling systems in L. enzymogenes strain OH11. By quantifying HSAF production levels in the 45 constructed mutants, we identified two strains that produced significantly smaller amounts of HSAF. One of the mutations affected a gene encoding a conserved bacterial response regulator, PilR, which is commonly associated with type IV pilus synthesis. We determined that L. enzymogenes PilR regulates pilus synthesis and twitching motility via a traditional pathway, by binding to the pilA promoter and upregulating pilA expression. Regulation of HSAF production by PilR was found to be independent of pilus formation. We discovered that the pilR mutant contained significantly higher intracellular levels of the second messenger cyclic di-GMP (c-di-GMP) and that this was the inhibitory signal for HSAF production. Therefore, the type IV pilus regulator PilR in L. enzymogenes activates twitching motility while downregulating antibiotic HSAF production by increasing intracellular c-di-GMP levels. This study identifies a new role of a common pilus regulator in proteobacteria and provides guidance for increasing antifungal antibiotic production in L. enzymogenes.

  • 相关文献

[1]LetR is a TetR family transcription factor from Lysobacter controlling antifungal antibiotic biosynthesis. Wang, Ping,Chen, Hongfu,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan.

[2]Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11. Wang, Ruping,Xu, Huiyong,Zhao, Yangyang,Liu, Fengquan,Wang, Ruping,Zhang, Juan,Qian, Guoliang,Liu, Fengquan,Yuen, Gary Y.. 2017

[3]Heat-Stable Antifungal Factor (HSAF) Biosynthesis in Lysobacter enzymogenes Is Controlled by the Interplay of Two Transcription Factors and a Diffusible Molecule. Su, Zhenhe,Liu, Fengquan,Su, Zhenhe,Han, Sen,Qian, Guoliang,Liu, Fengquan,Su, Zhenhe,Han, Sen,Qian, Guoliang,Liu, Fengquan,Fu, Zheng Qing. 2018

[4]Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11. Yu, Lingjun,Liu, Fengquan,Yu, Lingjun,Su, Wei,Du, Liangcheng,Fey, Paul D.. 2018

[5]产酶溶杆菌OH11代谢产物HSAF对梨树腐烂病的防治效果. 赵延存,李红旭,何锋,曹素芳,杨晓蕾,钱国良,刘凤权. 2017

[6]Transcriptional and Antagonistic Responses of Biocontrol Strain Lysobacter enzymogenes OH11 to the Plant Pathogenic Oomycete Pythium aphanidermatum. Zhao, Yangyang,Liu, Fengquan,Qian, Guoliang,Chen, Yuan,Du, Liangcheng. 2017

[7]LesR is a novel upstream regulator that controls downstream Clp expression to modulate antibiotic HSAF biosynthesis and cell aggregation in Lysobacter enzymogenes OH11. Xu, Huiyong,Zhao, Yangyang,Liu, Fengquan,Wang, Ruping,Qian, Guoliang,Fu, Zheng Qing. 2017

[8]Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11. Wang, Yansheng,Zhao, Yuxin,Zhang, Juan,Zhao, Yangyang,Shen, Yan,Su, Zhenhe,Xu, Gaoge,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan,Du, Liangcheng,Huffman, Justin M.,Venturi, Vittorio. 2014

[9]Production of Antifungal p-Aminobenzoic Acid in Lysobacter antibioticus OH13. Laborda, Pedro,Zhao, Yangyang,Ling, Jun,Hou, Rongxian,Liu, Fengquan. 2018

[10]Type IV pilus biogenesis genes and their roles in biofilm formation in the biological control agent Lysobacter enzymogenes OH11. Xia, Jing,Chen, Jiaojiao,Chen, Yuan,Qian, Guoliang,Liu, Fengquan,Liu, Fengquan. 2018

[11]ChpA Controls Twitching Motility and Broadly Affects Gene Expression in the Biological Control Agent Lysobacter enzymogenes. Zhou, Mimi,Shen, Danyu,Xu, Gaoge,Qian, Guoliang,Liu, Fengquan.

[12]Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6. Zhang, Qingxia,Xiao, Qi,Xu, Jingyou,Tong, Yunhui,Chen, Xijun,Wen, Jia,Wei, Lihui.

[13]Interactive Effects of Tetracyclines and Copper on Plant Growth and Nutrient Uptake by Eichhornia crassipes. Lu, Xin,Gao, Yan,Luo, Jia,Yan, Shaohua,Wang, Tong,Liu, Lizhu,Zhang, Zhenhua,Lu, Xin,Gao, Yan,Luo, Jia,Yan, Shaohua,Wang, Tong,Liu, Lizhu,Zhang, Zhenhua. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台