文献类型： 外文期刊
作者： Gao, Yi 1 ; Qin, Lihong 1 ; Yang, Yuwei 3 ; Dong, Xue 2 ; Zhao, Zijiao 2 ; Zhang, Guoliang 1 ; Zhao, Zhihui 3 ;
作者机构： 1.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling 136100, Jilin, Peoples R China
2.Jilin Agr Univ, Coll Anim Sci & Technol, Xin Cheng St 2888, Changchun 130118, Jilin, Peoples R China
3.Jilin Univ, Coll Anim Sci, Changchun 130062, Jilin, Peoples R China
关键词： PDPN;Sertoli cells;Proliferation
期刊名称：ANIMAL REPRODUCTION SCIENCE （ 2020影响因子：2.145； 五年影响因子：2.281 ）
收录情况： SCI
摘要： Podoplanin (PDPN) is a transmembrane receptor which is involved in various physiological and pathological processes, such as cell motility, invasion, tumor metastasis and blood vessels formation. Although there are reports on the involvement of PDPN in Sertoli cells in human and mice, the role of PDPN on the development of bovine Sertoli cells has not been reported. In the present study, Sertoli cells were isolated from 1-day-old bovine testes by two steps enzyme digestion method. Feulgen staining of satellite karyosomes and inhibin immunofluorescence staining suggested that the isolated immature Sertoli cells were very pure. Transfection with overexpression plasmid pBI-CMV3-PDPN and interference shRNA plasmid indicated that PDPN could significantly promote Sertoli cells cycle progression, cells proliferation and androgen-binding protein (ABP) production. Our results indicated that PDPN gene plays a significant role in the proliferation and maturation of bovine Sertoli cells. (C) 2017 Elsevier B.V. All rights reserved.
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