您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and characterization of a novel calreticulin involved in the immune response of the Zhikong scallop, Chlamys farreri

文献类型： 外文期刊

作者： Wang, Guanghua 1 ; Jiang, Zengjie 3 ; Yang, Ning 2 ; Zhu, Dongfa 1 ; Zhang, Min 2 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China

2.Qingdao Agr Univ, Marine Sci & Engn Coll, 700 Changcheng Rd, Qingdao 266109, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

4.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China

关键词： Chlamys farreri;Calreticulin;Bacteria-binding;Innate immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Calreticulin (CRT) is a multifunctional calcium-binding chaperone shared among vertebrates and invertebrates. In this study, a novel CRT (CfCRT) was identified in the Zhikong scallop Chlamys farreri by rapid amplification of cDNA ends. The full-length cDNA was composed of 1345 bp, which included a 1158 bp open reading frame, a 25 bp 5'-untranslated region (UTR) and a 162 bp 3'-UTR. The predicted molecular mass of CfCRT was 44.8 kDa. CfCRT contained three highly conserved domains (N-, P- and C-domains) essential to the function of CRT. BLAST analysis revealed significant sequence similarity (73%-92%) with CRT proteins from other mollusks. The mRNA transcripts of CfCRT were present in all the tested tissues of Zhikong scallops, with the higher expression level in the hemocytes and mantle. After stimulation by Vibrio anguillarum, the mRNA transcript of CfCRT in hemocytes was significantly upregulated. Recombinant plasmid pBCRT was successfully expressed in Escherichia coli BL21 (DE3). The recombinant (r)CfCRT protein could bind to the surface of several bacteria including the Gram-negative bacteria V. anguillarum, E. coli, and the Gram-positive bacterium Staphylococcus aureus. Moreover, rCfCRT was able to suppress their growth significantly. These results indicate that CfCRT might act as an immune effector in Zhikong scallop innate immunity. (C)2017 Published by Elsevier Ltd.

  • 相关文献

[1]Cloning and characterization of calreticulin and its association with salinity stress in P-trituberculatus. Lv, Jianjian,Wang, Yu,Zhang, Dening,Gao, Baoquan,Liu, Ping,Li, Jian,Wang, Yu,Zhang, Dening.

[2]Assortative fertilization in Chlamys farreri and Patinopecten yessoensis and its implication in scallop hybridization. Lu, Zhengming,Yang, Aiguo,Wang, Qingyin,Liu, Zhihong,Zhou, Liqing. 2006

[3]Variations in retention efficiency of bivalves to different concentrations and organic content of suspended particles. Zhang Jihong,Fang Jianguang,Liang Xingming. 2010

[4]Bioinformatics analysis of hemocyte miRNAs of scallop Chlamys farreri against acute viral necrobiotic virus (AVNV). Chen, Guofu,Zhang, Chunyun,Jiang, Fengjuan,Wang, Yuanyuan,Xu, Zhong,Wang, Chongming. 2014

[5]A novel serine protease with clip domain from scallop Chlamys farreri. Zhu, Ling,Song, Linsheng,Zhao, Jiangmin,Li, Chenghua,Xu, Wei,Zhu, Ling,Mao, Yuze. 2008

[6]Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure. Zhang Hui,Zhai Yuxiu,Yao Lin,Jiang Yanhua,Li Fengling,Zhang Hui,Zhai Yuxiu,Yao Lin,Jiang Yanhua,Li Fengling,Zhang Hui,Zhai Yuxiu,Yao Lin,Jiang Yanhua,Li Fengling. 2017

[7]Discovery of genes associated with cadmium accumulation from gill of scallop Chlamys farreri based on high-throughput sequencing. Zhang, Hui,Zhai, Yuxiu,Yao, Lin,Jiang, Yanhua,Li, Fengling,Zhang, Hui,Zhai, Yuxiu,Yao, Lin,Jiang, Yanhua,Li, Fengling,Zhang, Hui,Zhai, Yuxiu,Yao, Lin,Jiang, Yanhua,Li, Fengling.

[8]Carbon dioxide fixation by the seaweed Gracilaria lemaneiformis in integrated multi-trophic aquaculture with the scallop Chlamys farreri in Sanggou Bay, China. Han, Tingting,Han, Tingting,Han, Tingting,Jiang, Zengjie,Fang, Jianguang,Zhang, Jihong,Mao, Yuze,Zou, Jian,Huang, Yao,Wang, Dongzhe,Huang, Yao,Wang, Dongzhe.

[9]Molecular characterization and immune response expression of the QM gene from the scallop Chlamys farreri. Chen, Guofu,Zhang, Chunyun,Wang, Yue,Wang, Yuanyuan,Guo, Changlu,Wang, Chongming.

[10]Primary culture of Zhikong scallop Chlamys farreri hemocytes as an in vitro model for studying host-pathogen interactions. Ji, Aichang,Li, Xueyu,Fang, Sha,Qin, Zhenkui,Zhang, Zhifeng,Bai, Changming,Wang, Chongming.

[11]The effects of Zhikong scallop (Chlamys farreri) on the microbial food web in a phosphorus-deficient mariculture system in Sanggou Bay, China. Lu, Jiachang,Lu, Jiachang,Huang, Lingfeng,Xiao, Tian,Zhang, Wuchang,Jiang, Zengjie.

[12]Size fraction of phytoplankton and the contribution of natural plankton to the carbon source of Zhikong scallop Chlamys farreri in mariculture ecosystem of the Sanggou Bay. Jiang Zengjie,Du Meirong,Fang Jinghui,Gao Yaping,Li Jiaqi,Fang Jianguang,Jiang Zengjie,Fang Jianguang,Zhao Li. 2017

[13]A preliminary study of differentially expressed genes of the scallop Chlamys farreri against acute viral necrobiotic virus (AVNV). Chen, Guofu,Wang, Chenchao,Zhang, Chunyun,Wang, Yuanyuan,Xu, Zhong,Wang, Chongming. 2013

[14]Emerging and endemic types of Ostreid herpesvirus 1 were detected in bivalves in China. Bai, Changming,Wang, Chongming,Xia, Junyang,Zhang, Shuai,Huang, Jie,Bai, Changming,Wang, Chongming,Huang, Jie,Sun, Hailin.

[15]Physiological and biochemical responses of Zhikong scallop, Chlamys farreri, to different thermal stressors. Jiang, Weiwei,Jiang, Weiwei,Jiang, Weiwei,Jiang, Zengjie,Du, Meirong,Mao, Yuze,Li, Jiaqi,Fang, Jinghui,Lv, Xuning,Xue, Suyan,Wang, Wei,Zhang, Jihong,Fang, Jianguang,Jiang, Zengjie,Zhang, Jihong,Fang, Jianguang,Zhang, Yuan.

[16]Assessment of the local environmental impact of intensive marine shellfish and seaweed farming-Application of the MOM system in the Sungo Bay, China. Zhang, Jihong,Fang, Jianguang,Wang, Wei,Jiang, Zengjie,Zhang, Jihong,Fang, Jianguang,Wang, Wei,Jiang, Zengjie,Hansen, Pia Kupka.

[17]Modelling the effect of food depletion on scallop growth in Sungo Bay (China). Bacher, C,Grant, J,Hawkins, AJS,Fang, JG,Zhu, MY,Besnard, M.

[18]Effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, Cyprinus carpio. Chi, Cheng,Jiang, Bing,Yu, Xiao-Bo,Liu, Tian-Qiang,Wang, Gao-Xue,Xia, Lei. 2014

[19]Characterization of the Mx and hepcidin genes in Epinephelus akaara asymptomatic carriers of the nervous necrosis virus. Mao, Ming-Guang,Jiang, Jie-Lan,Wang, Ke-Jian,Lei, Ji-Lin,Lei, Ji-Lin,Mao, Ming-Guang,Lei, Ji-Lin,Peralvarez-Marin, Alex. 2013

[20]Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity. Yang, Lishi,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan,Qiu, Lihua,Huang, Jianhua,Jiang, Shigui,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台