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Developing insect resistance with fusion gene transformation of chitinase and scorpion toxin gene in maize (Zea mays L)

文献类型: 外文期刊

作者: Liu, Ming 1 ; Hao, Yaoshan 2 ; Sun, Yi 2 ; Wang, Jingxue 1 ;

作者机构: 1.Shanxi Univ, Sch Life Sci, Taiyuan 030006, Peoples R China

2.Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Shanxi Acad Agr Sci, Biotechnol Res Ctr, Taiyuan 030031, Peoples R China

关键词: maize;insect-resistant gene combination;chitinase gene;scorpion insect toxin gene;transgenic plant;calibrated mortality of larvae

期刊名称:MAYDICA ( 影响因子:0.542; 五年影响因子:0.605 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Transgenic plants with introduced pest-resistant genes provide an efficient alternative insect control. A binary insect-resistant gene combination, containing an insect-specific chitinase gene (chi) and a scorpion insect toxin gene (Bmk), was introduced into a maize cultivar via pollen-mediated transformation. Thirty-eight putative transgenic plantlets with kanamycin-resistance were obtained. Transgenic statuses of plants were confirmed by Southern blot analysis. Bioassay by inoculation of Asian corn borer (Ostrinia furnacalis Guenee; ACB) larvae indicated that the degree of ACB resistance varied among the transgenic plants. The highest average calibrated mortality of larvae was approximately 67%. The genetic analysis of T1 progeny confirmed that the inheritance of introduced genes followed the Mendelian's rules.

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