您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Lipopolysaccharide and heat stress impair the estradiol biosynthesis in granulosa cells via increase of HSP70 and inhibition of smad3 phosphorylation and nuclear translocation

文献类型： 外文期刊

作者： Li, Hui 1 ; Guo, Shuangshuang 2 ; Cai, Liuping 1 ; Ma, Weiming 2 ; Shi, Zhendan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Key Lab Anim Breeding & Reprod, Nanjing, Jiangsu, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Shandong, Peoples R China

关键词： Lipopolysaccharide;Heat stress;Granulosa cell;Estradiol;HSP70;Smad3

期刊名称：CELLULAR SIGNALLING （ 影响因子：4.315； 五年影响因子：4.206 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： LPS and heat stress have been shown to exert various toxic effects in animals, as they induce estradiol biosynthesis dysfunction in granulosa cells (GCs) and result in low reproductive performance. However, there is limited information regarding their detailed mechanisms. In the present study, primary cultured porcine GCs were treated with LPS (1000 ng/mL for 48 h), or heat stress (41 degrees C for 3 h), in vitro, with or without the HSP70 inhibitor VER155008 (10 mu M), to investigate their potential mechanisms. To mimic the spike in HSP70 from LPS and heat stress, treatments with only the HSP70 activator STA-4783 (10 mu M for 3 h or 48 h) were also performed. We found that LPS and heat stress treatments could significantly reduce the expressions of FSHR and CYP19A1; associated with a reduction in estradiol concentrations; and increased in HSP70 expression both at mRNA and protein levels. While, VER155008 attenuation of LPS and heat stress induced HSP70 upregulation can restore the expressions of FSHR and CYP19A1. Furthermore, STA-4783 treatment alone can mimic the effects of LPS and heat stress treatments. Following immunofluorescence staining and western blot analysis showed that Smad3 phosphorylation and nuclear translocation were also inhibited by LPS, heat stress and STA-4783 treatments. We also examined the interactions between HSP70 and Smad3 by yeast two-hybrid screening, the results revealed that HSP70 indirectly interacted with Smad3. Thus, our results suggested that LPS and heat stress could impair estradiol biosynthesis in GCs via increased HSP70 and indirect inhibition of Smad3 phosphorylation and nuclear translocation. (C) 2016 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Systemic analysis of gene expression profiles in porcine granulosa cells during aging. Li Hui,Guo Shuangshuang,Yu Jianning,Shi Zhendan. 2017

[2]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

[3]Improving Geese Production Performance in "Goose-Fish" Production System by Competitive Reduction of Pathogenic Bacteria in Pond Water. Shi Zhen-dan,Yang Xi-wen,Liu Li,Jiang Dan-li,Wang Cong-li,Sun Ai-dong. 2012

[4]Effects of dietary betaine supplementation subjected to heat stress on milk performances and physiology indices in dairy cow. Zhang, L.,An, W. J.,Lian, H.,Zhou, G. B.,Han, Z. Y.,Ying, S. J.. 2014

[5]Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress. Zhou, Rong,Zhao, Liping,Wang, Yinlei,Yu, Wengui,Zhao, Tongmin,Zhou, Rong,Zhao, Liping,Wang, Yinlei,Yu, Wengui,Zhao, Tongmin,Zhou, Rong,Yu, Xiaqing,Wu, Zhen,Ottosen, Carl-Otto,Rosenqvist, Eva. 2017

[6]Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress. Guo, Meng,Liu, Jin-Hong,Lu, Jin-Ping,Zhai, Yu-Fei,Wang, Hu,Gong, Zhen-Hui,Lu, Ming-Hui,Wang, Shu-Bin. 2015

[7]Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato. Ji, Chang Yoon,Jin, Rong,Kim, Ho Soo,Lee, Chan-Ju,Kang, Le,Kim, So-Eun,Kwak, Sang-Soo,Ji, Chang Yoon,Jin, Rong,Lee, Chan-Ju,Kang, Le,Kim, So-Eun,Kwak, Sang-Soo,Jin, Rong,Xu, Zhen,Xie, Yiping,Li, Hongmin,Ma, Daifu,Lee, Hyeong-Un,Lee, Joon Seol,Kang, Chang Ho,Chi, Yong Hun,Lee, Sang Yeol,Kang, Chang Ho,Chi, Yong Hun,Lee, Sang Yeol. 2017

[8]High throughput sequencing of herbaceous peony small RNAs to screen thermo-tolerance related microRNAs. Hao, Zhaojun,Liu, Ding,Hao, Zhaojun,Liu, Ding,Gong, Saijie,Zhao, Daqiu,Tao, Jun,Zhao, Daqiu,Tao, Jun.

[9]Protein sHSP26 improves chloroplast performance under heat stress by interacting with specific chloroplast proteins in maize (Zea mays). Hu, Xiuli,Yang, Yanfang,Gong, Fangping,Zhang, Li,Wu, Liuji,Li, Chaohao,Wang, Wei,Zhang, Dayong.

[10]De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress. Xu, Hai,Song, Bo,Chen, Jinfeng,Xu, Hai,Chen, Longzheng,Song, Bo,Fan, Xiaoxue,Yuan, Xihan.

[11]Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes. Hao, Zhaojun,Tao, Jun,Hao, Zhaojun,Wei, Mengran,Gong, Saijie,Zhao, Daqiu,Tao, Jun,Zhao, Daqiu,Tao, Jun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台