您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Isolation, cloning, and expression of five genes related to nitrogen metabolism in peach (Prunus persica L. Batsch)

文献类型： 外文期刊

作者： Zhang, C. H. 1 ; Zhang, B. B. 1 ; Yu, M. L. 1 ; Ma, R. J. 1 ; Song, Z. Z. 1 ; Korir, N. K. 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Inst Hort, 50 Zhongling St, Nanjing 210014, Peoples R China

2.Kenyatta Univ, Dept Agr Sci & Technol, POB 43844-00100, Nairobi, Kenya

关键词： Peach;Prunus persica;nitrogen metabolism;urea;gene expression

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： An optimal dosage of nitrogen has long been known to play important roles in governing nitrogen-use efficiency and improving the yield of plants. However, there is limited information on the isolation and expression profiles of genes related to nitrogen metabolism in peach (Prunus persica L. Batsch). This study isolated five genes involved in nitrogen metabolism and evaluated the effects of a single foliar application of urea on levels of expression of these genes in the leaves of 'Dazhenbaochiyue' peach. Cloning resulted in the isolation of 1,767, 1,236, 1,074, 1,758, and 2,721 nt-long cDNAs with full-length open reading frames encoding 588, 411, 357, 585, and 906 amino acids representing the asparagine synthetase (AS), glutamate dehydrogenase (GDH), glutamine synthetase (GS), nitrite reductase (NiR), and nitrate reductase (NR) genes in peach, respectively. An alignment of multiple amino acid sequences revealed that the AS, GDH, GS, and NR proteins in peach shared high levels of sequence conservation or identity at the amino acid level with their homologues in Arabidopsis thaliana and grapevine (Vitis vinifera). Based on analyses of expression of the five genes in peach leaves, we demonstrated that genes related to nitrogen metabolism responded to a foliar application of urea within 2 d. This was consistent with previous reports which indicated that leaves rapidly absorbed urea-nitrogen after foliar application. Foliar application of 0.5% (w/v) urea inhibited expression of GS and NiR, but increased expression of GDH, AS, and NR compared to control leaves. The different patterns of expression of the five genes in this study suggested that their expression might reflect their various roles in nitrogen metabolism, plant N status, and responses to weather conditions such as high light intensity, temperature, or humidity, all of which affect photosynthesis. These findings provide a basis for future functional analyses of these five genes in peach.

  • 相关文献

[1]Effects of the fungal endophyte Phomopsis liquidambari on nitrogen uptake and metabolism in rice. Yang, Bo,Wang, Xiao-Mi,Ma, Hai-Yan,Jia, Yong,Dai, Chuan-Chao,Li, Xia.

[2]Transcription of potassium transporter genes of KT/HAK/KUP family in peach seedlings and responses to abiotic stresses. Song, Z. -Z.,Yang, Y.,Ma, R. -J.,Xu, J. -L.,Yu, M. -L.,Song, Z. -Z.,Yang, Y.,Ma, R. -J.,Xu, J. -L.,Yu, M. -L.,Song, Z. -Z..

[3]Cloning and expression of genes related to the sucrose-metabolizing enzymes and carbohydrate changes in peach. Zhang, Chunhua,Shen, Zhijun,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Korir, Nicholas Kibet.

[4]Isolation and expression analysis of four HD-ZIP III family genes targeted by microRNA166 in peach. Zhang, C. H.,Zhang, B. B.,Ma, R. J.,Yu, M. L.,Guo, S. L.,Guo, L.. 2015

[5]叶面喷施不同浓度ABA对美香桃果实品质的影晌. 许建兰,马瑞娟,张斌斌,倪林箭. 2012

[6]Rearrangement of nitrogen metabolism in rice (Oryza sativa L.) under salt stress. Xu, Jianwen,Huang, Xi,Lan, Hongxia,Zhang, Hongsheng,Huang, Ji,Xu, Jianwen.

[7]Effects of 5-aminolevulinic acid on nitrogen metabolism and ion distribution of watermelon seedlings under salt stress. Chen, G.,Fan, P. S.,Feng, W. M.,Guan, A. Q.,Lu, Y. Y.,Wan, Y. L..

[8]Improvement of nitrogen accumulation and metabolism in rice (Oryza sativa L.) by the endophyte Phomopsis liquidambari. Yang, Bo,Ma, Hai-Yan,Wang, Xiao-Mi,Jia, Yong,Hu, Jing,Dai, Chuan-Chao,Li, Xia.

[9]Calcium involved in the poly(gamma-glutamic acid)-mediated promotion of Chinese cabbage nitrogen metabolism. Xu, Zongqi,Lei, Peng,Feng, Xiaohai,Liang, Jinfeng,Chi, Bo,Xu, Hong,Xu, Zongqi,Lei, Peng,Feng, Xiaohai,Liang, Jinfeng,Chi, Bo,Xu, Hong,Xu, Xianju,Feng, Xiaohai,Liang, Jinfeng,Chi, Bo,Xu, Hong. 2014

[10]Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Zhang, Yanping,Yu, Huaping,Han, Jian,Song, Changnian,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan.

[11]Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering. Song, Z. -Z.,Zhang, B. -B.,Zhang, C. -H.,Ma, R. -J.,Yu, M. -L.,Song, Z. -Z.,Zhang, B. -B.,Zhang, C. -H.,Ma, R. -J.,Yu, M. -L..

[12]Inbreeding and coancestry of the major commercial fresh market peach cultivars in China. Ma, Ruijuan,Yu, Mingliang,Du, Ping,Shen, Zhijun,Byrne, David H.. 2006

[13]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[14]Effect of salicylic acid on freezing injury in peach floral organs and the expressions of CBF genes. Ma, R. J.. 2017

[15]Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach. Shen, Zhijun,Yu, Mingliang,Ma, Ruijuan,Confolent, Carole,Lambert, Patrick,Poessel, Jean-Luc,Quilot-Turion, Benedicte,Pascal, Thierry. 2013

[16]Genome-wide analysis and identification of KT/HAK/KUP potassium transporter gene family in peach (Prunus persica). Song, Z. Z.,Ma, R. J.,Yu, M. L.. 2015

[17]The PpLTP1 Primary Allergen Gene is Highly Conserved in Peach and Has Small Variations in Other Prunus Species. Zhou, Xiang,Gao, Zhong-shan,Li, Xiong-wei,Jia, Hui-juan,Wu, Hong-xia,Xie, Rang-jin,Gao, Zhong-shan,Wang, Zhi-qiang,Cao, Ke,Yu, Ming-liang,Chen, Shuang-jian,Li, Ying-hui,Wang, Hui-ying,van Ree, Ronald.

[18]Controlled-release fertilizer, floating duckweed, and biochar affect ammonia volatilization and nitrous oxide emission from rice paddy fields irrigated with nitrogen-rich wastewater. Sun, Haijun,Sun, Haijun,Min, Ju,Feng, Yanfang,Shi, Weiming,Zhang, Hailin,Feng, Yanfang.

[19]Structure, expression profile, and evolution of the sucrose synthase gene family in peach (Prunus persica). Zhang, Chunhua,Yu, Mingliang,Ma, Ruijuan,Shen, Zhijun,Zhang, Binbin,Korir, Nicholas Kibet.

[20]KT/HAK/KUP potassium transporter genes differentially expressed during fruit development, ripening, and postharvest shelf-life of 'Xiahui6' peaches. Song, Zhizhong,Guo, Shaolei,Zhang, Chunhua,Zhang, Binbin,Ma, Ruijuan,Yu, Mingliang,Song, Zhizhong,Guo, Shaolei,Zhang, Chunhua,Zhang, Binbin,Ma, Ruijuan,Yu, Mingliang,Korir, Nicholas Kibet.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台