Preparative Isolation of 5 Antioxidant Constituents from the Medicinal Mushroom Phellinus baumii (Agaricomycetes) by High-Speed Countercurrent Chromatography and Preparative High-Performance Liquid Chromatography
文献类型: 外文期刊
作者: Ye, Libin 1 ; Zheng, Hongjian 3 ; Zhang, Zhong 1 ; Yang, Yan 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Inst Edible Fungi, Shanghai, Peoples R China
2.Peoples Republ China Minist Agr, Key Lab Edible Fungi Resources & Utilizat South, Shanghai, Peoples R China
3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou, Zhejiang, Peoples R China
关键词: antioxidant;HSCCC;isolation;medicinal mushrooms;Phellinus baumii;radical scavenger
期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )
ISSN:
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收录情况: SCI
摘要: Four extract fractions from the fruiting bodies of Phellinus baumii were acquired. Preliminary investigation of radical scavenging ability showed that the ethyl acetate extract exhibited the strongest antioxidant activity. To gain insight into the antioxidant mechanism, 5 antioxidant constituents were prepared from the ethyl acetate extract using high-speed countercurrent chromatography with a solvent system consisting of n-hexane, ethyl acetate, methanol, and water (0.8: 3: 1.5: 2.5, v/v/v/v),and preparative high-performance liquid chromatography. Electrospray ionization mass spectrometry and nuclear magnetic resonance spectra were used to analyze the structures of these compounds and revealed that they were caffeic acid (compound 1), inoscavin A (compound 2), 3,4-dihydroxy benzaldehyde (compound 3), coumarin (compound 4), and naringenin (compound 5). Compound 2 showed strong antioxidant activity, and compounds 1, 3, and 5 showed moderate antioxidant activity. Thus, a more clearly and usefully scientific background is provided herein for the development of relative functional foods or biomedical products using Ph. baumii as the raw material.
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