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Label-free and rapid detection of ATP based on structure switching of aptamers

文献类型： 外文期刊

作者： Ji, Danyang 1 ; Wang, Hongqi 2 ; Ge, Jia 1 ; Zhang, Lin 1 ; Li, Jianjun 1 ; Bai, Dongmei 1 ; Chen, Juan 1 ; Li, Zhaohui 1 ;

作者机构： 1.Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Peoples R China

2.Henan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhengzhou 450002, Peoples R China

3.Hunan Univ, Inst Chem Biol & Nanomed, Changsha 410082, Hunan, Peoples R China

关键词： ATP detection;Aptamer;Thioflavin T;Label-free;Fluorescence

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

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收录情况： SCI

摘要： In this work, an aptamer-based fluorescent strategy for label-free detection of ATP was developed by using Thioflavin T (ThT) as a fluorescence indicator, which can specifically bind with G-quadruplex DNAs to generate enhanced fluorescence intensity. In the absence of ATP, the folded structure of ATP aptamer allows the intercalation of ThT to produce strong fluorescence signal. However, upon ATP binding to the aptamer where ThT intercalated, the conformational change or distortion of the aptamer is large enough to cause much less intercalation of ThT and consequently drastic suppression of the fluorescence intensity. As such, the concentration of ATP could be identified very easily by observing fluorescence changes of this sensing system. This label-free assay could be accomplished very easily and quickly with a "mix-and-detect" detection method and exhibits high sensitivity to ATP with a detection limit of 33 nM in a wide range of 0.1-1000 mu M. Furthermore, this proposed method is capable of detecting ATP in human serum and cell extracts. This method offers several advantages such as simplicity, rapidity, low cost, good stability and excellent selectivity, which make it hold great potential for the detection of ATP in bioanalytical and biological studies. (C) 2017 Elsevier Inc. All rights reserved.

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