您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Oxyradical stress increases the biosynthesis of 2-arachidonoylglycerol: involvement of NADPH oxidase

文献类型： 外文期刊

作者： Matthews, Anberitha T. 1 ; Lee, Jung Hwa 1 ; Borazjani, Abdolsamad 1 ; Mangum, Lee C. 1 ; Hou, Xiang 1 ; Ross, Matt 1 ;

作者机构： 1.Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Ctr Environm Hlth Sci, Mississippi State, MS 39762 USA

2.Jiangsu Acad Agr Sci, Inst Food Safety, Nanjing, Jiangsu, Peoples R China

关键词： 2-arachidonoylglycerol;macrophages;NADPH oxidase;atherosclerosis;endocannabinoids

期刊名称：AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY （ 影响因子：4.249； 五年影响因子：4.627 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： NADPH oxidase (Nox)-derived oxyradicals contribute to atherosclerosis by oxidizing low-density lipoproteins (LDL), leading to their phagocytosis by vascular macrophages. Endocannabinoids, such as 2-arachidonoylglycerol (2-AG), might be an important link between oxidative stress and atherosclerosis. We hypothesized that 2-AG biosynthesis in macrophages is enhanced following ligation of oxidized LDL by scavenger receptors via a signal transduction pathway involving Nox-derived ROS that activates diacylglycerol lipase-beta (DAGL-beta), the 2-AG biosynthetic enzyme. To test this idea, we challenged macrophage cell lines and murine primary macrophages with a xanthine oxidase system or with nonphysiological and physiological Nox stimulants [phorbol 12-my-ristate 13-acetate (PMA) and arachidonic acid (AA)]. Each stressor increased cellular superoxide levels and enhanced 2-AG biosynthetic activity in a Nox-dependent manner. Levels of cytosolic phospho-lipase A_2-dependent AA metabolites (eicosanoids) in primary macrophages were also dependent on Nox-mediated ROS. In addition, 2-AG levels in DAGL-beta-overexpressing COS7 cells were attenuated by inhibitors of Nox and DAGL-beta. Furthermore, ROS induced by men-adione (a redox cycling agent) or PMA could be partially attenuated by the cannabinoid 1/2 receptor agonist (WIN 55,212-2). Finally, cells that overexpress Nox2 components (Phox-COS7) synthesized larger amounts of 2-AG compared with the parental COS7 cells. Together, the results suggest a positive correlation between heightened oxygen radical flux and 2-AG biosynthesis in macrophage cell lines and primary macrophages. Because of the antioxidant and anti-inflammatory effects associated with 2-AG, the increased levels of this bioae-tive lipid might be an adaptive response to oxidative stress. Thus oxyradical stress may be counteracted by the enhanced endocannabi-noid tone.

  • 相关文献

[1]Promotion of atherosclerosis in high cholesterol diet-fed rabbits by immunization with the P277 peptide. Xiong, Qiyan,Feng, Jiao,Zhang, Yu,Sun, Yunxiao,Lu, Yong,Li, Taiming,Cao, Rongyue,Jin, Liang,Wu, Jie,Xiong, Qiyan,Feng, Jiao,Jin, Liang,Zhang, Xiaohong.

[2]Identification of palmitoyl protein thioesterase 1 in human thp1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Wang, Ran,Borazjani, Abdolsamad,Matthews, Anberitha T.,Mangum, Lee C.,Ross, Matthew K.,Wang, Ran,Edelmann, Mariola J..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台