Expression Differences of Resistance-Related Genes Induced by Cycloxaprid Using qRT-PCR in the Female Adult of Sogatella furcifera (Hemiptera: Delphacidae)
文献类型： 外文期刊
作者： Jin, Jian-Xue 1 ; Jin, Dao-Chao 1 ; Li, Feng-Liang 2 ; Cheng, Ying 2 ; Li, Wen-Hong 2 ; Ye, Zhao-Chun 2 ; Zhou, Yu-Han 1 ;
作者机构： 1.Guizhou Univ, Inst Entomol, Prov Key Lab Agr Pest Management Mountainous Reg, Guiyang 550025, Guizhou, Peoples R China
2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550006, Guizhou, Peoples R China
关键词： Sogatella furcifera (Horvath);P450;decarboxylase;glycosyltransferase;qRT-PCR
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）
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收录情况： SCI
摘要： As a newer cis-nitromethylene neonicotinoid pesticide at present, cycloxaprid has good industrialization prospects, including the management of imidacloprid-resistant populations, because this chemical have an excellent efficiency against rice planthoppers. Sogatella furcifera (Horvath) is the most economically important pest of rice worldwide and has developed resistance to many insecticides. This study focused on the expression change of these resistance genes, induced by cycloxaprid, involved in metabolic detoxification and receptor protein. Twenty-two differentially expressed genes (DEGs) that may be related with the insecticide resistance were found in the transcriptome of S. furcifera, including 2 cytochrome P450 genes, 2 glutathione S-transferase (GST) genes, 1 acid phosphatase (ACP) gene, 12 decarboxylase genes, 2 glycolipid genes, 1 cadherin gene, and 2 glycosyltransferase genes, which were up- or downregulated in response to an exposure of cycloxaprid. Furthermore, two P450 genes (CYP4 and CYP6 family, respectively), two decarboxylase genes, and one glycosyltransferase gene were validated by qRT-PCR. Expression differences of these genes verified successfully by qRT-PCR in response to different concentrations and times treated with cycloxaprid could explain the insecticide resistance mechanism under cycloxaprid stress in S. furcifera.
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