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Transcriptome sequencing ofCoccinella septempunctataadults (Coleoptera: Coccinellidae) feeding on artificial diet andAphis craccivora

文献类型： 外文期刊

作者： Cheng, Ying 1 ; Zhi, Junrui 1 ; Li, Fengliang 2 ; Wang, Hua 3 ; Zhou, Yuhang 2 ; Jin, Jianxue 2 ;

作者机构： 1.Guizhou Univ, Inst Entomol, Guizhou Prov Key Lab Agr Pest Management Mt Reg, Guiyang, Peoples R China

2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

3.Guizhou Inst Forest Inventory & Planning, Guiyang, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 8 期

页码：

收录情况： SCI

摘要： Background The insect predatorCoccinella septempunctatacan effectively control many types of pests, such as aphids, whiteflies, and small lepidopteran larvae. We previously found thatC.septempunctatafed an artificial diet showed diminished biological properties(e.g. fecundity, egg hatching rate, survival rate, etc.) compared with those fed natural prey (Aphis craccivora), likely due to different nutritional characteristics of the diet. In this study, we used transcriptome sequencing analysis to identify nutrition- and metabolism-related genes ofC.septempunctatathat were differentially expressed depending on diet. Methodology/Principal findings The Illumina HiSeq2000 was used to sequence 691,942,058 total clean reads from artificial diet-fed andA.craccivora-fedC.septempunctatalibraries, and the clean reads were assembled using Trinityde novosoftware (Tabel2). Comparison of transcriptome sequences revealed that expression of 38,315 genes was affected by the artificial diet, and 1,182 of these genes showed a significant change in expression levels (FDR <= 0.05,|log2FC|>= 1, "FC" stands for "fold change"). These differentially expressed genes (DEGs) were likely associated with the decreased egg laying capacity, hatching rate, longevity, and increased sex ratio (female:male) of adultC.septempunctataobserved in the group fed the artificial diet. Furthermore, in the most DEGs metabolic pathways forC.septempunctatafeeding on the artificial diet accumulated amino acid metabolic pathways, lipid metabolic pathways, and starch and glucose metabolism were down-regulated. Conclusions/Significance We found some differentially expressed genes and metabolic pathways are related to nutrition, from which a more informative feedback for diet formulation was obtained and the artificial diet could be more efficiently optimized.

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