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Growth performance, reproductive traits and offspring survivability of genetically modified rams overexpressing toll-like receptor 4

文献类型： 外文期刊

作者： Yao, Yu-Chang 1 ; Han, Hong-Bing 2 ; Song, Xu-Ting 1 ; Deng, Shou-Long 2 ; Liu, Yu-Feng 4 ; Lu, Ming-Hai 5 ; Zhang, Yu 1 ;

作者机构： 1.Northeast Agr Univ, Coll Anim Sci & Technol, 59 Mucaijie, Harbin 150030, Peoples R China

2.China Agr Univ, Beijing Key Lab Anim Genet Improvement, Beijing, Peoples R China

3.Educ Dept Heilongjiang Prov, Key Lab Anim Genet Breeding & Reprod, Harbin, Peoples R China

4.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin, Peoples R China

5.Heilongjiang State Farms Sci Technol V

关键词： Toll-like receptor 4;Genetically modified sheep;Growth performance;Reproductive traits;Offspring survivability

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Genetic modification provides a means to enhancing disease resistance in animals. Toll-like receptor 4 (TLR4), a member of the TLR family, is critical for the recognition of lipopolysaccharide (LPS)/endotoxin from Gram-negative bacteria by host immune cells, which initiates cell activation and subsequently triggers a proinflammatory response to the invading pathogens. In this study, the first generation of genetically modified (GM) sheep overexpressing TLR4 was produced by microinjection for better disease resistance. Compared with wild-type (WT) rams, the GM rams have similar growth performance, basic semen quality and spermatozoon ultrastructure. The offspring birth rates after cervical artificial insemination were also similar between GM (90.32%) and WT (92.38%) rams. Overall, the presence and expression of the TLR4 transgene in the genome did not appear to interfere with normal semen production, reproductive traits and the ability of transgene transmission to offspring. The expression levels of TLR4, tumor necrosis factor and interferon gamma genes in monocyte/macrophages from GM sheep were significantly higher than that from WT sheep at early stages after LPS stimulation. The GM offspring born from the founder transgenic ram inseminated ewes had similar survival rate with WT offspring (88.89% vs 84.86%) at weaning. The TLR4 transgene showed no deleterious effects on growth performance, reproductive traits and offspring survivability of GM rams. Therefore, the GM sheep over expressing TLR4 provide a powerful experimental model for analyzing function of TLR4 in vivo during infection and inflammation. (C) 2017 Elsevier Inc. All rights reserved.

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