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Long-term cryopreservation had no adverse effect on viability of embryos and their offspring in sheep

文献类型： 外文期刊

作者： Yao, Yu-Chang 1 ; Qi, Mei-Yu 2 ; Lu, Ming -Hai 3 ; Wang, Su-Mei 4 ; Li, Wu 1 ; Han, Hong-Bing 5 ;

作者机构： 1.NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin, Peoples R China

3.Vocat Inst Technol Heilongjiang Land Reclamat, Dept Anim Sci, Harbin, Peoples R China

4.Heilongjiang Vocat Coll Sci & Technol, Dept Anim Sci & Technol, Shuangcheng, Peoples R China

5.China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China

关键词： Sheep;Embryo;Cryopreservation;Viability

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2012 年 136 卷 1-2 期

页码：

收录情况： SCI

摘要： Cryopreservation has been widely utilized in livestock and human embryos, which allows for storage of worthy embryos for a long period of time, although it is still uncertain as how long embryos can be cryopreserved in liquid nitrogen. The aims of this study were to evaluate the effects of long-term cryopreservation on birth rate of transferred sheep embryos at morula or blastocyst stage, and to investigate growth performance and viability of their offspring. A total of 373 sheep embryos from the same batch, which had been cryopreserved by conventional procedure for 0.5 yr (n = 259) or 7.5 yr (n = 114), respectively, were transferred to 373 recipient ewes. In parallel, artificial inseminations, acting as controls, were conducted in the same month in both years (n = 81 and n = 110) that embryo transfers were performed. Results showed that there were no significant differences in birth rate between short-term cryopreservation group (cryopreserved for 0.5 yr in 2003) and long-term cryopreservation group (cryopreserved for 7.5 yr in 2010) either at the morula or blastocyst stage (p > 0.05). No specific differences in birth weight were observed among short-term cryopreservation, artificial insemination 1 (performed in 2003), long-term cryopreservation and artificial insemination 2 (performed in 2010) group (p > 0.05). The weaning weights were similar between the short-term cryopreservation and long-term cryopreservation group (p > 0.05). The mortality rates of the offspring were similar in both groups as well (p > 0.05). We concluded that the long-term cryopreservation did not appear to adversely affect birth rate of the embryos, growth performance and viability of their offspring. Our results indicated that the cryopreserved sheep embryos should be stable in liquid nitrogen for at least 7.5 years. (C) 2012 Elsevier B.V. All rights reserved.

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