您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Ultrasensitive fluorometric glutathione assay based on a conformational switch of a G-quadruplex mediated by silver(I)

文献类型： 外文期刊

作者： Ji, Danyang 1 ; Meng, Hongmin 1 ; Ge, Jia 1 ; Zhang, Lin 1 ; Wang, Hongqi 2 ; Bai, Dongmei 1 ; Li, Jianjun 1 ; Qu, Lingbo 1 ;

作者机构： 1.Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Henan, Peoples R China

2.Henan Acad Agr Sci, Inst Qual Standard & Testing Technol Agroprod, Zhengzhou 450002, Henan, Peoples R China

3.Hunan Univ, Inst Chem Biol & Nanomed, Changsha 410082, Hunan, Peoples R China

关键词： Methylmesoporphyrin IX;G-quadruplex;Fluorescence;Quenching;Stern-Volmer plot;Cell extracts

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The authors describe a silver(I) mediated fluorescent assay for glutathione (GSH). An allosteric oligonucleotide strand containing a G-rich sequence is used to produce a G-quadruplex, and N-methylmesoporphyrin IX (NMM) is chosen as the fluorescent probe. In the absence of Ag(I), the DNA strand is partially intramolecularly hybridized to form a hairpin structure wherein the G-rich sequence is partially caged. On addition of Ag(I), the hairpin is disrupted by forming C-Ag(I)-C base pairs. As a result, the G-rich sequence is released and folds into a G-quadruplex structure, which is able to bind NMM to generate strong fluorescence at 612 nm. However, in the presence of GSH, due to the strong binding ability between GSH and Ag(I), the C-Ag(I)-C structure is not formed. Hence, the DNA probe reverts back to its original structure and fluorescence is not increased. Based on these findings, a method was worked out that has a detection limit as low as 3.5 nM. Due to the inherent selectivity of the interaction between GSH and Ag(I), the method is highly selective over common potential interfering species. It was successfully applied to the fluorometric determination of GSH in cell extracts.

  • 相关文献

[1]A Turn on ESIPT Probe for Rapid and Ratiometric Fluorogenic Detection of Hg2+ and its Application in Live-Cell Imaging. Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng,Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng,Zhang, Di,Liu, Jihong,Yin, Haiyan,Wang, Hongqi,Li, Shufang,Wang, Min,Li, Man,Zhou, Ling,Zhang, Junfeng. 2016

[2]An ESIPT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution and its Application in Living Cells. Ju, Zhiyu,Li, Dapeng,Li, Dandan,Wu, Changzeng,Xu, Zhihong,Zhang, Di. 2017

[3]Label-free and rapid detection of ATP based on structure switching of aptamers. Ji, Danyang,Ge, Jia,Zhang, Lin,Li, Jianjun,Bai, Dongmei,Chen, Juan,Li, Zhaohui,Wang, Hongqi,Li, Zhaohui.

[4]An aptamer-based signal-on bio-assay for sensitive and selective detection of Kanamycin A by using gold nanoparticles. Chen, Jing,Li, Zhaohui,Ge, Jia,Yang, Ran,Zhang, Lin,Qu, Ling-bo,Qu, Ling-bo,Wang, Hong-qi,Zhang, Ling.

[5]Two rhodamine based chemosensors for Sn4+ and the application in living cells. Cheng, Junye,Yang, Erbing,Ding, Peigang,Tang, Jun,Zhang, Di,Zhao, Yufen,Ye, Yong,Zhang, Di,Zhao, Yufen,Ye, Yong.

[6]Highly sensitive and fast responsive ratiometric fluorescent probe for Cu2+ based on a naphthalimide-rhodamine dyad and its application in iving cell imaging. Tang, Jun,Ma, Saige,Liu, Yaqi,Zhao, Yufen,Ye, Yong,Zhang, Di,Zhao, Yufen.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台