您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells

文献类型： 外文期刊

作者： Qiu, Yueqin 1 ; Ma, Xianyong 1 ; Yang, Xuefen 1 ; Wang, Li 1 ; Jiang, Zongyong 2 ;

作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Key Lab Anim Nutr & Feed Sci South China,Minist A, Guangdong Publ Lab Anim Breeding & Nutr,Inst Anim, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding, Key Lab Anim Nutr & Feed Sci South China,Minist A, Guangdong Publ Lab Anim Breeding & Nutr,I

关键词： Sodium butyrate;IPEC-J2 cells;Cell proliferation;Cell cycle arrest;Apoptosis

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0.5 and 1 mM) of butyrate had no effect on cell proliferation. However, at 5 and 10 mM, sodium butyrate significantly decreased cell viability, accompanied by reduced levels of p-mTOR and PCNA protein. Sodium butyrate, in a dose-dependent manner, induced cell cycle arrest in G0/G1 phase and reduced the numbers of cells in S phase. In addition, relative expression of p21, p27, and pro-apoptosis bak genes, and protein levels of p21Waf1/Cip1, p27Kip1, cyclinD3, CDK4, and Cleave-caspase3 were increased by higher concentrations of sodium butyrate (1, 5, 10 mM), and the levels of cyclinD1 and CDK6 were reduced by 5 and 10 mM butyrate. Butyrate increased the phosphorylated form of the signaling molecule p38 and phosphorylated JNK. In conclusion, the present in vitro study indicated that sodium butyrate inhibited the proliferation of IPEC-J2 cells by inducing cell cycle arrest in the G0/G1 phase of cell cycles and by increasing apoptosis at high concentrations.

  • 相关文献

[1]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[2]MiR-34b-5p Suppresses Melanoma Differentiation-Associated Gene 5 (MDA5) Signaling Pathway to Promote Avian Leukosis Virus Subgroup J (ALV-J)-Infected Cells Proliferaction and ALV-J Replication. Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan,Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan,Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan. 2017

[3]The multi-herbal formula Chong-Myung-Tang improves spatial memory and increases cell genesis in the dentate gyrus of aged mice. Liu, Lei,Zhang, Mingwei,Zhang, Ruifen,Lee, Mira,Hou, Jingang,Sung, Chang-Keun,Wang, Zhen. 2014

[4]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[5]Effects of dietary glycyl-glutamine on growth performance, small intestinal integrity, and immune responses of weaning piglets challenged with lipopolysaccharide. Jiang, Z. Y.,Lin, Y. C.,Ma, X. Y.,Zheng, C. T.,Zhou, G. L.,Chen, F.,Zou, S. T.,Sun, L. H..

[6]CpG oligodeoxynucleotides augment the immune responses of piglets to swine Pasteurella multocida living vaccine in vivo. Zhang Linghua,Tian Xingshan,Zhou Fengzhen.

[7]Conjugated linoleic acid differentially regulates fat deposition in backfat and longissimus muscle of finishing pigs. Jiang, Z. Y.,Zhong, W. J.,Zheng, C. T.,Lin, Y. C.,Jiang, S. Q.,Zhong, W. J.,Yang, L..

[8]Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials. Cui, Jianghu,Yang, Yunhua,Zheng, Mingtao,Liu, Yingliang,Xiao, Yong,Lei, Bingfu,Chen, Wei. 2014

[9]Role of gga-miR-221 and gga-miR-222 during Tumour Formation in Chickens Infected by Subgroup J Avian Leukosis Virus. Dai, Zhenkai,Yan, Yiming,Lin, Wencheng,Li, Hongxin,Chen, Feng,Liu, Yang,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Dai, Zhenkai,Yan, Yiming,Lin, Wencheng,Li, Hongxin,Chen, Feng,Liu, Yang,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Ji, Jun,Xie, Qingmei,Li, Hongxin,Chen, Feng,Xie, Qingmei,Chen, Feng,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Chen, Feng,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei. 2015

[10]Autophagy in Lepidoptera: more than old wine in new bottle. Tettamanti, G.,Grimaldi, A.,de Eguileor, M.,Cao, Y.,Feng, Q.. 2011

[11]Transcriptomic analysis reveals differentially expressed genes and a unique apoptosis pathway in channel catfish ovary cells after infection with the channel catfish virus. Dawar, Farman Ullah,Hu, Xianqin,Dong, Xingxing,Xiong, Yang,Mei, Jie,Lin, Li,Dawar, Farman Ullah,Zhao, Lijuan,Zhou, Meng,Liang, Rishen,Babu, V. Sarath,Lin, Li,Hu, Xianqin,Li, Jun,Li, Jun,Lin, Li,Lin, Li.

[12]BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation. Xie, Kun,Tian, Ling,Guo, Xinyu,Li, Kang,Li, Jianping,Deng, Xiaojuan,Zhong, Yangjin,Huang, Zhijun,Liu, Jiping,Yang, Wanying,Cao, Yang,Xie, Kun,Tian, Ling,Li, Kang,Li, Sheng,Li, Qingrong,Xia, Qingyou.

[13]Dietary vitamin A supplementation improved reproductive performance by regulating ovarian expression of hormone receptors, caspase-3 and Fas in broiler breeders. Chen, Fang,Chen, Fang,Jiang, Zongyong,Jiang, Shouqun,Li, Long,Lin, Xiajing,Gou, Zongyong,Fan, Qiuli.

[14]Deficient and excess dietary selenium levels affect growth performance, blood cells apoptosis and liver HSP70 expression in juvenile yellow catfish Pelteobagrus fulvidraco. Hu, Jun-Ru,Wu, Ying-Xia,Xian, Jian-An,Wang, An-Li,Hu, Jun-Ru,Huang, Yan-Hua,Wang, Guo-Xia,Cao, Jun-Ming.

[15]Protective Role of Selenium on Aflatoxin B-1-Induced Hepatic Dysfunction and Apoptosis of Liver in Ducklings. Liao, Shenquan,Shi, Dayou,Guo, Shining,Su, Rongsheng,Qiang, Panjia,Tang, Zhaoxin,Shi, Dayou,Guo, Shining,Su, Rongsheng,Qiang, Panjia,Tang, Zhaoxin,Shi, Dayou,Guo, Shining,Su, Rongsheng,Qiang, Panjia,Tang, Zhaoxin,Clemons-Chevis, Connie L..

[16]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[17]The core structure of a Dendrobium huoshanense polysaccharide required for the inhibition of human lens epithelial cell apoptosis. Zha, Xue-Qiang,Zha, Xue-Qiang,Li, Xiao-Long,Wang, Jing-Fei,Pan, Li-Hua,Luo, Jian-Ping,Deng, Yuan-Yuan.

[18]miR-16 controls myoblast proliferation and apoptosis through directly suppressing Bcl2 and FOXO1 activities. Nie, Qinghua.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台