A simple and efficient non-denaturing FISH method for maize chromosome differentiation using single-strand oligonucleotide probes
文献类型： 外文期刊
作者： Zhu, Minqiu 1 ; Du, Pei 1 ; Zhuang, Lifang 1 ; Chu, Chenggen 2 ; Zhao, Han 3 ; Qi, Zengjun 1 ;
作者机构： 1.Nanjing Agr Univ, Agr Coll, Nanjing 210095, Jiangsu, Peoples R China
2.Monsanto Co, 21120 Hwy 30, Filer, ID 83301 USA
3.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China
关键词： chromosome diversity;non-denaturing FISH;oligonucleotide;Zea mays
期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）
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收录情况： SCI
摘要： Single-strand oligonucleotides (SSONs hereafter) as probes are becoming a powerful method of chromosome painting in many species. In this study, nine SSONs ((ACT)(10), (ACT)(19), Knob-1, Knob-2, Knob-3, CentC69-1, MR68-3, K10-72-1, and TR1-357-2) were developed and used for chromosome identification in 16 maize (Zea mays L., 2n = 20) inbred lines and hybrids by non-denaturing fluorescence in situ hybridization (ND-FISH). Each SSON produced clear signals on 2-10 chromosomes of inbred lines B73 and Mo17. A multiplex probe set containing four SSONs ((ACT) 10, Knob-2, CentC69-1, and MR68-3) clearly characterized all maize chromosomes in the 16 lines by a single round of ND-FISH and revealed genetic variation at a chromosome level. For example, unique signals on chromosome 6 clearly distinguished all 16 genotypes. The SSONs and multiplex probe developed in this research will facilitate genotype identification and chromosome research in maize.
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