您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Prevalence, Enterotoxin Gene and Antimicrobial Resistance of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Clinical Healthy Dairy Cows

文献类型： 外文期刊

作者： Bao, Hongduo 1 ; Zhang, Hui 1 ; Zhou, Yan 1 ; Zhang, Lili 1 ; Wang, Ran 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Key Lab Agrofood Safety & Qual Monitoring Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： Antimicrobial resistance;coa gene;Coagulase-positive S. aureus (CPS);MRSA;Raw milk;Staphylococcal enterotoxin (SE) genes

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Coagulase- positive Staphylococcus aureus (CPS) is a leading cause of both clinical and subclinical bovine mastitis. Moreover, methicillin- resistant Staphylococcus aureus (MRSA) have been identified as an emerging mastitis pathogen in dairy cows. In our study, 121 raw milk samples were collected from individual clinical healthy cows from five commercial farms. 52 samples (42.98%) were positively detected as CPS. Fifty-two strains of CPS were isolated and identified using phenotypic and molecular approaches. Their coagulase gene (coa gene) amplification showed a single amplicon of a size between 600 and 1000 bp. Five CPS isolated strains from YZ farm, which was resistant to oxacillin and contained a specific 310-bp for the mecA gene, were identified as MRSA. The staphylococcal enterotoxin a (SEa) gene was detected in 18 strains. None of the isolates carried the gene SEb, SEc, SEd and SEe. All isolates including the five MRSA isolates were uniformly susceptible to Cephalothin (KF), Ofloxacin (OFX) and Vancomycin hydrochloride (VA) at minimum inhibitory concentrations (MIC). Besides P and CIP, the resistance of these isolates resistances to Oxacillin (OX), rifampicin (RIF), Cefotaxime (CTX), Azithromyein (AZM) and Clindamycin (DA) were relatively low (<20%). Together, our findings demonstrated that there was high prevalence of CPS and ten MRSA contaminations in raw milk from clinical healthy cows. (C)2016 PVJ. All rights reserved

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台