Prevalence, Enterotoxin Gene and Antimicrobial Resistance of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Clinical Healthy Dairy Cows
文献类型： 外文期刊
作者： Bao, Hongduo 1 ; Zhang, Hui 1 ; Zhou, Yan 1 ; Zhang, Lili 1 ; Wang, Ran 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Key Lab Agrofood Safety & Qual Monitoring Agr, Nanjing 210014, Jiangsu, Peoples R China
关键词： Antimicrobial resistance;coa gene;Coagulase-positive S. aureus (CPS);MRSA;Raw milk;Staphylococcal enterotoxin (SE) genes
期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Coagulase- positive Staphylococcus aureus (CPS) is a leading cause of both clinical and subclinical bovine mastitis. Moreover, methicillin- resistant Staphylococcus aureus (MRSA) have been identified as an emerging mastitis pathogen in dairy cows. In our study, 121 raw milk samples were collected from individual clinical healthy cows from five commercial farms. 52 samples (42.98%) were positively detected as CPS. Fifty-two strains of CPS were isolated and identified using phenotypic and molecular approaches. Their coagulase gene (coa gene) amplification showed a single amplicon of a size between 600 and 1000 bp. Five CPS isolated strains from YZ farm, which was resistant to oxacillin and contained a specific 310-bp for the mecA gene, were identified as MRSA. The staphylococcal enterotoxin a (SEa) gene was detected in 18 strains. None of the isolates carried the gene SEb, SEc, SEd and SEe. All isolates including the five MRSA isolates were uniformly susceptible to Cephalothin (KF), Ofloxacin (OFX) and Vancomycin hydrochloride (VA) at minimum inhibitory concentrations (MIC). Besides P and CIP, the resistance of these isolates resistances to Oxacillin (OX), rifampicin (RIF), Cefotaxime (CTX), Azithromyein (AZM) and Clindamycin (DA) were relatively low (<20%). Together, our findings demonstrated that there was high prevalence of CPS and ten MRSA contaminations in raw milk from clinical healthy cows. (C)2016 PVJ. All rights reserved
- 相关文献
作者其他论文 更多>>
-
Activating plant immunity: the hidden dance of intracellular Ca2+ stores
作者：Wang, Qi;Yan, Haiqiao;Zhang, Zilu;Li, Wei;He, Jinyu;Lou, Laiqing;Chang, Ming;Cang, Xiaoyan;Zhang, Meixiang;Wang, Ran
关键词：Ca2+ signal; Ca2+ store; chloroplast; cytoskeleton; ER; mitochondrion; organelle; plant immunity
-
Soil Acidification Can Be Improved under Different Long-Term Fertilization Regimes in a Sweetpotato-Wheat Rotation System
作者：Zhang, Huan;Fu, Weiguo;Wang, Jidong;Zhang, Huan;Wang, Lei;Xu, Cong;Zhang, Hui;Xu, Xianju;Ma, Hongbo;Wang, Jidong;Zhang, Yongchun
关键词：soil acidification; sweetpotato-wheat rotation system; soil quality improvement; long-term fertilization; organic fertilization; arid-environment eco-restoration and sustainable agriculture construction
-
Biochar increases pakchoi yield by regulating soil bacterial communities but reduces it through soil fungi in vegetable soil
作者：Zhong, Lei;Gu, Zhibin;Wang, Ruying;Wang, Hongyue;Li, Gaoyuan;Sun, Yuru;Xiao, Hui;Zhang, Hui
关键词：High nitrogen fertilizer; Biochar; Soil microbial diversity; Yield; Agroecosystem
-
High-affinity truncated aptamers for detection of Cronobacter spp with magnetic separation-assisted DNAzyme-driven 3D DNA walker
作者：Yang, Ningru;Ding, Ning;Qi, Shuo;Shang, Zixuan;Ma, Pengfei;Khan, Imran Mahmood;Wang, Zhouping;Xia, Yu;Khan, Imran Mahmood;Wang, Zhouping;Zhang, Yin;Zhang, Lili;Wang, Zhouping
关键词：Cronobacter spp.; Aptamer; Truncation; DNA walker; Au nanoparticles; Fluorescence detection
-
Combining GC-MS/MS with a LLE-SPE sample pretreatment step to simultaneously analyse enrofloxacin and ofloxacin residues in chicken tissues and pork
作者：Pang, Maoda;Wang, Ran;Xu, Junjie;Tang, Yayun;Guo, Yawen;Ding, Hao;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Xie, Xing;Xie, Xing
关键词：Chicken tissues; Pork; Enrofloxacin; Ofloxacin; GC-MS/MS
-
Diverse Genotypes of Cronobacter spp. Associated with Dairy Farm Systems in Jiangsu and Shandong Provinces in China
作者：Liu, Hui;Ji, Xing;Sun, Haichang;Hou, Xiang;Soleimani-Delfan, Abbas;Wang, Ran;Zhang, Lili;Liu, Hui;Sun, Haichang;Zhang, Lili;Billington, Craig;Wang, Heye
关键词：Cronobacter spp.; whole-genome sequencing; antimicrobial susceptibility; phylogenetic analysis
-
Effective and food-grade detoxification of multiple mycotoxins using yeast expressed manganese peroxidases
作者：You, Yang;Qiu, Yangyu;Xu, Huidong;He, Rui;Wang, Zhouping;Xia, Yu;Zhang, Lili
关键词：Mycotoxins; Manganese peroxidase; Degradation; Pichia pastoris; Food-grade
