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MiR-144 affects fatty acid composition by regulating ELOVL6 expression in duck hepatocytes

文献类型： 外文期刊

作者： He, Jun 1 ; Tian, Yong 1 ; Zhao, Yan 2 ; Liu, Yali 1 ; Tao, Zhengrong 1 ; Li, Guoqin 1 ; Niu, Dong 3 ; Lu, Lizhi 1 ; Lu, Yin 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Wenzhou Vocat Coll Sci & Technol, Dept Anim Sci, Wenzhou 325006, Peoples R China

3.Zhejiang Univ, Coll Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China

4.Wenzhou Med Univ, Affiliated Hosp 1, Dept Emergency Med, Wenzhou 325000, Peoples R China

关键词： duck;ELOVL6;hepatocyte;lipid metabolism;microRNA;miR-144

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

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收录情况： SCI

摘要： Lipid metabolism in duck is very important for both raisers and people's health. In our previous studies, we have detected that miR-144 is related to duck lipid metabolism and validated one of its target genes, elongation of very long chain fatty acids protein 6 (ELOVL6). In the present study, we isolated, cultured, and identified duck hepatocytes, and transfected with miR-144 mimics/ inhibitor to mediate the miR-144 level. The qRT-PCR results showed that the ELOVL6 expression in duck hepatocytes was down/ upregulated, respectively. The fat contents and each fatty-acid percent content of the hepatocytes and medium were also determined. When ELOVL6 expression suppressed (miR-144 mimics transfected), the palmitic acid (C16: 0) content was significantly increased (P< 0.05); the oleic acid (C18: 1, n-9), eicosenoic acid (C20: 1, n-9), and eicosatrienoic acid (C20: 3) contents were significantly reduced (P< 0.05). The myristic acid (C14: 0) and palmitic acid (C16: 0) contents were significantly reduced (P< 0.05), and the oleic acid (C18: 1, n-9) content was significantly increased (P< 0.05) when ELOVL6 expression upregulated (miR-144 inhibitor transfected). It indicated that miR-144 could regulate some saturated fatty acids elongated to longer unsaturated fatty acids through controlling ELOVL6 expression. Whereas, miR-144/ ELOVL6 appeared not associated with fat deposition in duck hepatocytes (P> 0.05). Our findings suggest that miR-144 might regulate the percentages of fatty acids in duck hepatocytes through affecting ELOVL6 expression.

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