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Development of Serotype-Specific PCR Assays for Typing of Haemophilus parasuis Isolates Circulating in Southern China

文献类型： 外文期刊

作者： Jia, Aiqing 1 ; Zhou, Ruyue 2 ; Fan, Huiying 1 ; Yang, Kaijie 1 ; Zhang, Jianmin 1 ; Xu, Yindi 3 ; Wang, Guiping 2 ; Liao 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Key Lab Vet Vaccine Innovat, Minist Agr, Guangzhou, Guangdong, Peoples R China

2.Guangdong Haid Inst Anim Husb & Vet, Guangzhou, Guangdong, Peoples R China

3.Henan Acad Agr Sci, Inst Anim Husb & Vet Res, Zhengzhou, Henan, Peoples R China

关键词： serotyping;serotype-specific PCR;indirect hemagglutination assay;Haemophilus parasuis

期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 2020影响因子：5.948； 五年影响因子：5.345 ）

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收录情况： SCI

摘要： The bacterium Haemophilus parasuis is the specific pathogenic cause of Glssser's disease in swine. Fifteen serotypes of H. parasuis have been reported. A method to serotype H. parasuis isolates accurately would help to prevent and control Glasser's disease outbreaks through appropriate vaccination and to understand the epidemiology in specific geographic areas. However, according to traditional serotyping, the rate of nontypeable (NT) strains is 10 to 40%, which gives low accuracy. In the present study, we developed a set of PCR assays that are able to identify all the currently known H. parasuis serotypes, with a detection limit of 5 CFU. This PCR method is particularly useful to distinguish serotype 5 from serotype 12. We then surveyed the serotype prevalence of H. parasuis isolates from southern China using both the traditional indirect hemagglutination (IHA) and current PCR methods. Of the 298 isolates tested, 228 (76.51%) and 281 (94.30%) were serotyped by the IHA and PCR tests, respectively, with a concordance rate of 80.87% (241/298). The most prevalent serotypes obtained by PCR were 4, 5, 12, 13, NT, and 2, and the most prevalent obtained by IHA were NT, 5, 4, 12, 13, and 2. In conclusion, the PCR assays developed in this study provide a rapid and specific method for the molecular serotyping of H. parasuis.

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