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Generation and characterization of caprine chymosin in corn seed

文献类型： 外文期刊

作者： Liu, Wen-Guo 1 ; Wang, Yun-Peng 1 ; Zhang, Zhi-Jun 1 ; Wang, Min 2 ; Lv, Qing-Xue 2 ; Liu, Hong-Wei 1 ; Meng, Ling-Con 1 ;

作者机构： 1.Jilin Acad Agr Sci, 1363 Shengtai St, Changchun 130124, Jilin Province, Peoples R China

2.Jilin Jinong Hitech Inc Ltd, Gong Zhu Ling 136100, Peoples R China

关键词： Corn seed;Caprine chymosin;Transgenic plants

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

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收录情况： SCI

摘要： Abstract Chymosin is widely used in the dairy industry, and much is produced through recombinant DNA in organisms such as bacteria and tobacco. In this study, we used a new transgenic method to express caprine chymosin in corn seeds with lower cost and better storage capability. The recombinant chymosin protein was successfully expressed at an average level of 0.37?mg/g dry weight, which is 0.27% of the total soluble protein in the corn seed. Prochymosin can be activated to produce a chymosin protein with the ability to induce clotting in milk, similar to the commercial protein. The activity of the purified recombinant chymosin was as high as 178.5?U/mg. These results indicate that we have successfully established a technology for generating corn seed-derived caprine chymosin for potential use in the dairy industry. Highlights ? The paper was the first report of the production of caprine chymosin in field food crop. ? The recombinant chymosin protein was successfully expressed and activated in the corn seed. ? The new method to express chymosin has advantages of lower cost and better storage capability. ? This corn seed-derived caprine prochymosin shows promise for use in the dairy industry. ]]>

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