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PxAPN5 serves as a functional receptor of Cry2Ab in Plutella xylostella (L.) and its binding domain analysis

文献类型： 外文期刊

作者： Pan, Zhi-Zhen 1 ; Xu, Lian 2 ; Liu, Bo 1 ; Zhang, Jing 2 ; Chen, Zheng 1 ; Chen, Qing-Xi 2 ; Zhu, Yu-Jing 1 ;

作者机构： 1.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Fujian, Peoples R China

2.Xiamen Univ, Sch Life Sci, Key Lab, Minist Educ Coastal & Wetland Ecosyst, Xiamen 361102, Fujian, Peoples R China

关键词： Bacillus thuringiensis;Cry2Ab;Plutella xylostella;AminopeptidaseN 5;Binding region

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： Lepidopteran midgut aminopeptidases N (APNs) are widely studied for their potential roles as one of the receptors for Bacillus thuringiensis (Bt) crystal toxins. In the present study, a loss of function analyses by RNAi (RNA interference) silencing of the Plutella xylostella APN5 (PxAPN5), a binding protein of Bt crystal toxin Cry2Ab, were performed. The knocking down of PxAPN5 in P. xylostella larvae greatly reduced their susceptibility to Cry2Ab and led to a decrease of Cry2Ab binding to P. xylostella midgut. Four truncated fragments of PxAPN5 were further constructed and expressed in Escherichia coli (E.coli) to find the binding region of PxAPN5 to Cry2Ab. The ligand blot result indicated that D1 domain (residues 1-262) and D3 domain (residues 510-620) of PxAPN5 could specially bind to Cry2Ab. (C) 2017 Elsevier B.V. All rights reserved.

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[1]Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.. Pan, Zhizhen,Zhu, Yujing,Shi, Huai,Chen, Zheng,Chen, Meichun,Liu, Bo,Xu, Lian,Chen, Qingxi.

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