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Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

作者： Li, Huawei 1 ; Yang, Jifei 2 ; Bao, Dengke 2 ; Hou, Jie 2 ; Zhi, Yubao 2 ; Yang, Yanyan 2 ; Ji, Pengchao 1 ; Zhou, Enmin; 1 ;

作者机构： 1.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhenzhou 450002, Peoples R China

4.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

5.Henan Univ An

关键词： enzyme-linked immunosorbent assay;immunochromatography;porcine reproductive and respiratory syndrome

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.

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[1]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[2]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[3]Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine. Li, Xiujie,Sun, Yaning,Yang, Suzhen,Wang, Yinbiao,Yang, Jifei,Liu, Yunchao,Jin, Qianyue,Li, Xuewu,Guo, Chengliu,Zhang, Gaiping,Zhang, Gaiping,Li, Xiujie,Yang, Suzhen,Zhang, Gaiping.

[4]Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2. Jin, Qianyue,Wang, Yinbiao,Chen, Wen,Jin, Qianyue,Yang, Jifei,Lu, Qingxia,Guo, Junqing,Deng, Ruiguang,Wang, Yinbiao,Wang, Shihong,Wang, Shufen,Chen, Wen,Zhi, Yubao,Wang, Li,Yang, Suzhen,Zhang, Gaiping,Wang, Shufen,Wang, Shufen. 2012

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