Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS-LRR gene ZmNL in maize (Zea mays)
文献类型： 外文期刊
作者： Di, Hong 1 ; Yu, Tao 2 ; Deng, Yanxue 1 ; Dong, Xiaojie 1 ; Li, Rou 1 ; Zhou, Yu 1 ; Wang, Zhenhua 1 ;
作者机构： 1.Northeast Agr Univ, Harbin 150030, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Maize Inst, Postdoctoral Workstn, Harbin 150086, Heilongjiang, Peoples R China
关键词： ZmNL gene;Transgenic maize;Resistance to head smut disease;(NBS)-leucine-rich repeat (LRR) gene family
期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）
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收录情况： SCI
摘要： The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family comprises the largest number of known disease resistance (R) genes and is one of the largest gene families in plants. In the present study, the full-length cDNA of ZmNL (GenBank Accession Number KF765443) was isolated using Rapid Amplification of cDNA Ends. The nucleotide sequence of ZmNL contains an open reading frame of 3156 bp that encodes the ZmNL protein, which is comprised of 1051 amino acid residues. This putative protein has high homology to other known resistance proteins (84% to Triticum aestivum LR10) and belongs to the CC-NBS-LRR type R gene family. The ZmNL gene was introduced into the maize inbred line of Huangzao4 which was highly susceptible to head smut under the control of the maize ubiquitin promoter by Agrobacterium-mediated transformation. The head smut disease incidence of 3 T-2 transgenic lines was significantly reduced (by 18.38-29.40%) compared with the wild type, which indicated that the overexpression of ZmNL gene in maize enhanced the resistance to the fungus Sporisorium reilianum (Kuhn) Clint of these plants.
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