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The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis

文献类型： 外文期刊

作者： Wang, Jingqian 1 ; Liu, Zhao 1 ; Gao, Xinming 1 ; Du, Chen 1 ; Hou, Congcong 1 ; Tang, Daojun 1 ; Lou, Bao 2 ; Shen, Weiliang 3 ; Zhu, Junquan 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.Ningbo Acad Oceanol & Fishery, Ningbo 315012, Zhejiang, Peoples R China

关键词： Larimichthys crocea; Spermiogenesis; KIF17; Spermatid remodeling

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：3.014； 五年影响因子：3.187 ）

ISSN： 0920-1742

年卷期： 2022 年 48 卷 3 期

页码：

收录情况： SCI

摘要： KIF17, which belongs to the kinesin-2 protein family, plays an indispensable role in mammalian spermiogenesis. However, the role of KIF17 in fish spermatid remodeling during spermiogenesis remains poorly understood. Therefore, we aimed to study the role of KIF17 in spermatid remodeling during Larimichthys crocea (L. crocea) spermiogenesis. The kif17 cDNA sequence, 3247 bp in length, was cloned from L. crocea testis, which consisted of a 347-bp 5 '-untranslated region (UTR), 413-bp 3 ' -UTR, and 2487-bp open reading frame. Bioinformatic analyses revealed that KIF17 obtained from L. crocea (Lc-KIF17) exhibited a high sequence identity compared with those from other teleosts and possessed the structural features of other kinesin-2 proteins. Based on structural similarity, we speculate that the role of Lc-KIF17 may be similar to that of KIF17 in other animals. Lc-kif17 mRNA was diffusely expressed in L. crocea tissues and was highly expressed in the testis, especially at stage IV testicular development. Immunofluorescence analysis revealed that Lc-KIF17 signals colocalized with beta-tubulin signals and migrated from the perinuclear cytoplasm to the side of the nucleus where the tail forms during spermiogenesis. These findings revealed that KIF17 may be involved in L. crocea spermiogenesis. In particular, KIF17 may participate in spermatid remodeling by interacting with perinuclear microtubules during L. crocea spermiogenesis. Collectively, this study contributes to an improved understanding of the mechanism underlying L. crocea spermiogenesis and provides a basis for further research on L. crocea reproduction and development.

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