文献类型: 外文期刊
作者: Gong, Qiong 1 ; Wang, Chaonan 2 ; Fan, Weiqiang 3 ; Li, Shuiling 1 ; Zhang, Hong 3 ; Huang, Zhiyin 2 ; Liu, Xiaohui 2 ; Ma, Ziyun 5 ; Wang, Yong 1 ; Zhang, Bin 2 ;
作者机构: 1.Nankai Univ, Coll Life Sci, Weijin Rd 94, Tianjin 300071, Peoples R China
2.Tianjin Acad Agr Sci, Vegetable Res Inst, Tianjin 300381, Peoples R China
3.Tianjin Acad Agr Sci, State Key Lab Vegetable Biobreeding, Tianjin 300192, Peoples R China
4.Vegetable Res Inst, Tianjin Kernel Agr Sci & Technol Co Ltd, Tianjin 300381, Peoples R China
5.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China
关键词: radish; pithiness; NADPH oxidase; respiratory burst oxidase homologs (Rbohs)
期刊名称:PLANTS-BASEL ( 影响因子:4.5; 五年影响因子:4.8 )
ISSN: 2223-7747
年卷期: 2024 年 13 卷 10 期
页码:
收录情况: SCI
摘要: Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes.
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