文献类型： 外文期刊

作者： Liu, Zhengqun ¹ ; Wang, Han ¹ ; Han, Hui ⁴ ; Li, Ning ¹ ; Zheng, Zi ¹ ; Liang, Shiyue ¹ ; Zhong, Ruqing ⁴ ; Chen, Liang ⁴ ; Yan, Jun ¹ ; Mu, Shuqin ¹ ;

作者机构： 1.Tianjin Acad Agr Sci, Tianjin Key Lab Anim Mol Breeding & Biotechnol, 82 Jinjing Rd, Tianjin 300381, Peoples R China

2.Tianjin Acad Agr Sci, Tianjin Engn Res Ctr Anim Hlth Farming, 82 Jinjing Rd, Tianjin 300381, Peoples R China

3.Inst Anim Sci & Vet, Tianjin Acad Agr Sci, 82 Jinjing Rd, Tianjin 300381, Peoples R China

4.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr & Feeding, Beijing, Peoples R China

5.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China

6.Southwest Univ Sci & Technol, Tianfu Inst Res & Innovat, Chengdu, Sichuan, Peoples R China

关键词： Dulcitol; Intestinal barrier function; Gut microbiota; TLR4/ NF-kappa B kappa B signaling pathway; Apoptosis; Piglets

期刊名称：JOURNAL OF NUTRITIONAL BIOCHEMISTRY （ 影响因子：4.9； 五年影响因子：5.5 ）

ISSN： 0955-2863

年卷期： 2024 年 133 卷

页码：

收录情况： SCI

摘要： This study investigated the protective effect of dulcitol on LPS-induced intestinal injury in piglets and explored the underlying molecular mechanisms. A total of 108 piglets were divided into three groups: CON, LPS, and DUL. The CON and LPS groups were fed a basal diet, the DUL group was fed a diet supplementation with 500 mg/kg dulcitol. On day 29, 6 piglets in the LPS and DUL groups were injected with 100 mu g/kg BW of LPS. At 4 h postchallenge, all pigs were slaughtered, and colonic samples were collected. Results showed that dulcitol supplementation boosted intestinal barrier function in LPSchallenged piglets by enhancing intestinal morphology and integrity, and increasing the gene expression of zonula occludens-1, claudin-1, , and occludin in the colonic mucosa ( P < 0.05). Metabolomics showed DUL supplementation mainly increased ( P < 0.05) the metabolites related to steroid and vitamin metabolism (Cholesterol and Vitamin C). Proteomics showed that dulcitol supplementation altered the protein expression involved in maintaining barrier integrity (FN1, CADM1, and PARD3), inhibiting inflammatory response (SLP1, SFN, and IRF3), and apoptosis (including FAS, ING1, BTK, MTHFR, NOX, and P53BP2) in LPS-challenged piglets ( P < 0.05). Additionally, dulcitol addition also suppressed the TLR4/NF-kappa B kappa B signaling pathway and apoptosis in mRNA and protein levels. Dulcitol increased the abundance of short-chain fatty acid-producing bacteria ( Lactobacillus, Blautia, , and Faecalibacterium) at the genus level, but decreased the relative abundance of Proteobacteria at the phylum level and Pseudomonas and Delftia at the genus level in piglets ( P < .05). In conclusion, these results suggested that the addition of dulcitol alleviated LPS-induced intestinal barrier injury in piglets, probably by maintaining its integrity, inhibiting the TLR4/NF-kappa B kappa B signaling pathways and apoptosis, and modulating the gut microbiota. Therefore, dulcitol can be considered a potential dietary additive for improving intestinal health in pig models.