Copper chelating peptides derived from tilapia (Oreochromis niloticus) skin as tyrosinase inhibitor: Biological evaluation, in silico investigation and in vivo effects
文献类型： 外文期刊
作者： Song, Yuqiong 1 ; Li, Jun 1 ; Tian, Han 3 ; Xiang, Huan 1 ; Chen, Shengjun 1 ; Li, Laihao 1 ; Hu, Xiao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
2.Chinese Acad Sci, RNAM Ctr Marine Microbiol South China Sea Inst Oce, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
3.Shanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R China
4.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
5.Dalian Polytech Univ, Collaborat Innovat Ctr Prov & Ministerial Coconstr, Dalian 116034, Peoples R China
关键词： Tilapia (Oreochromis niloticus) skin; Copper chelating peptides; Tyrosinase inhibition; Molecular dynamics
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）
ISSN： 0963-9969
年卷期： 2023 年 163 卷
页码：
收录情况： SCI
摘要： Binuclear copper ions at the active site determine the catalysis of tyrosinase (TYR)1 whose activity can be inhibited by copper's chelation with other compounds. In this study, tilapia (Oreochromis niloticus) skin was used to generate TYR-inhibitory peptides after being treated by different enzymes and 4 h-Alcaline protease hydrolysate exhibited the highest TYR inhibition and copper chelation. Immobilized metal affinity chromatog-raphy was used for purifying copper chelating peptides, among which PFRMY (IC50: 0.43 +/- 0.08 mg/mL) and RGFTGM (IC50: 1.61 +/- 0.04 mg/mL) exhibited the highest TYR-inhibitory capacity and the lowest docking energy. Both two peptides inhibited TYR in a mixed manner and interacted with key residues binding to copper ions within TYR mainly by hydrogen bonds and hydrophobic forces, while PFRMY had a more compact and stable conjugation with TYR. Zebrafish assay revealed that PFRMY reduced not only melanin synthesis but in vivo TYR activity.
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