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Transcriptomic and Metabolomic Analyses Provide Insights into the Formation of the Peach-like Aroma of Fragaria nilgerrensis Schlecht. Fruits

文献类型: 外文期刊

作者: Wang, Ai-Hua 1 ; Ma, Hong-Ye 1 ; Zhang, Bao-Hui 1 ; Mo, Chuan-Yuan 1 ; Li, En-Hong 3 ; Li, Fei 1 ;

作者机构: 1.Guizhou Acad Agr Sci, Hort Inst, Guizhou Hort Engn Technol Res Ctr, Guiyang 550006, Peoples R China

2.Suzhou Univ, Coll Biol & Food Engn, Suzhou 234099, Peoples R China

3.Guizhou Seed Management Stn, Guiyang 550001, Peoples R China

关键词: Fragaria nilgerrensis Schlecht.; transcriptome; peach aroma; fatty acid metabolism; delta-octalactone; lactone biosynthesis

期刊名称:GENES ( 影响因子:4.141; 五年影响因子:4.474 )

ISSN:

年卷期: 2022 年 13 卷 7 期

页码:

收录情况: SCI

摘要: Fragaria nilgerrensis Schlecht. is a wild diploid strawberry species. The intense peach-like aroma of its fruits makes F. nilgerrensis an excellent resource for strawberry breeding programs aimed at enhancing flavors. However, the formation of the peach-like aroma of strawberry fruits has not been comprehensively characterized. In this study, fruit metabolome and transcriptome datasets for F. nilgerrensis (HA; peach-like aroma) and its interspecific hybrids PA (peach-like aroma) and NA (no peach-like aroma; control) were compared. In total, 150 differentially accumulated metabolites were detected. The K-means analysis revealed that esters/lactones, including acetic acid, octyl ester, delta-octalactone, and delta-decalactone, were more abundant in HA and PA than in NA. These metabolites may be important for the formation of the peach-like aroma of F. nilgerrensis fruits. The significantly enriched gene ontology terms assigned to the differentially expressed genes (DEGs) were fatty acid metabolic process and fatty acid biosynthetic process. Twenty-seven DEGs were predicted to be associated with ester and lactone biosynthesis, including AAT, LOX, AOS, FAD, AIM1, EH, FAH, ADH, and cytochrome P450 subfamily genes. Thirty-five transcription factor genes were predicted to be associated with aroma formation, including bHLH, MYB, bZIP, NAC, AP2, GATA, and TCPfamily members. Moreover, we identified differentially expressed FAD, AOS, and cytochrome P450 family genes and NAC, MYB, and AP2 transcription factor genes that were correlated with delta-octalactone and delta-decalactone. These findings provide key insights into the formation of the peach-like aroma of F. nilgerrensis fruits, with implications for the increased use of wild strawberry resources.

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