Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method
文献类型： 外文期刊
作者： Zhao, Binan 4 ; Yang, Dan 5 ; Zhang, Yongchun 1 ; Xu, Yan 3 ; Zhao, Xiao 1 ; Liang, Jieling 5 ; Fan, Xiaorui 3 ; Du, Yana 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
3.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China
4.XuZhou Med Univ, Dept Clin Med, Xuzhou, Peoples R China
5.Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China
关键词： LAMP;ornamental plant;Potyvirus;virus detection
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2018 年 163 卷 2 期
页码：
收录情况： SCI
摘要： Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMoV, with no cross-reaction with other lily viruses. The sensitivity of LMoV using the LAMP assay was 100 times more sensitive than that using conventional polymerase chain reaction. A reverse transcription LAMP (RT-LAMP) was then successfully applied to detect LMoV RNA. The newly established LAMP and one-step RT-LAMP provide an alternative method for detecting LMoV in lily plants.
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