您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

In Vitro Glucuronidation of Ochratoxin A by Rat Liver Microsomes

文献类型： 外文期刊

作者： Han, Zheng 1 ; Tangni, Emmanuel K. 2 ; Di Mavungu, Jose Diana 3 ; Vanhaecke, Lynn 4 ; De Saeger, Sarah 3 ; Wu, Aibo 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai 201403, Peoples R China

2.Vet & Agrochem Res Ctr CODA CERVA, Unit Toxins & Nat Components, B-3080 Tervuren, Belgium

3.Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

4.Univ Ghent, Fac Vet Med, Chem Anal Lab, B-9820 Merelbeke, Belgium

关键词： ochratoxin A;glucuronidation;metabolic pathway;rat liver microsomes;Orbitrap

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2013 年 5 卷 12 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MSn) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with -glucuronidase. Moreover, OTA methyl ester, OT and OT-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.

  • 相关文献

[1]Improving Rice Ability to Degrade Different Polycyclic Aromatic Hydrocarbons Through Multigene Transformation of a Hybrid Dioxygenase System. Peng, Ri-He,Shi, Biao,Fu, Xiao-Yan,Tian, Yong-Sheng,Zhao, Wei,Zhu, Bo,Xu, Jing,Han, Hong-Juan,Yuan, Zhong-Yi,Liu, Kai-Hong,Yao, Quan-Hong.

[2]Quantitative assessment of risk associated with dietary intake of mycotoxin ochratoxin A on the adult inhabitants in Shanghai city of PR China. Han, Zheng,Nie, Dongxia,Yang, Xianli,Wang, Jianhua,Wu, Aibo,Peng, Shaojie,Han, Zheng. 2013

[3]Combinatorial approach of LC-MS/MS and LC-TOF-MS for uncovering in vivo kinetics and biotransformation of ochratoxin A in rat. Han, Zheng,Zhao, Zhiyong,Zhao, Zhihui,Wu, Aibo,Shi, Jianxin,Zhang, Dabing,Liao, Yucai,Wu, Yongning,Han, Zheng,De Saeger, Sarah.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台