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Detection limit of Clostridium botulinum spores in dried mushroom samples sourced from China

文献类型： 外文期刊

作者： Malakar, Pradeep K. 1 ; Plowman, June 1 ; Aldus, Clare F. 1 ; Xing, Zengtao 2 ; Zhao, Yong 3 ; Peck, Michael W. 1 ;

作者机构： 1.Inst Food Res, Norwich NR4 7UA, Norfolk, England

2.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai 201401, Peoples R China

3.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

关键词： Dried mushrooms;China;Non-proteolytic Clostridium botulinum;Proteolytic Clostridium botulinum

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.277； 五年影响因子：5.388 ）

ISSN： 0168-1605

年卷期： 2013 年 166 卷 1 期

页码：

收录情况： SCI

摘要： A survey of dried mushrooms (Lentinula edodes (Shiitake) and Auricularia auricula (Wood Ear)) sourced from China was carried out to determine the natural contamination of these mushrooms with spores of proteolytic Clostridium botulinum and non-proteolytic C. botulinum. The mushrooms were collected from supermarkets and retailers in 21 cities in China during October 2008. Spore loads of C. botulinum in mushrooms have a degree of uncertainty and variability and this study contributes valuable data for determining prevalence of spores of C. botulinum in mushrooms. An optimized detection protocol that combined selective enrichment culture with multiplex PCR was used to test for spores of proteolytic and non-proteolytic C. botulinum. Detection limits were calculated, using a maximum likelihood protocol, from mushroom samples inoculated with defined numbers of spores of proteolytic C. botulinum or non-proteolytic C. botulinum. Based on the maximum likelihood detection limit, it is estimated that dried mushroom A. auricula contained <550 spores/kg of proteolytic C botulinum, and <350 spores/kg of non-proteolytic C botulinum. Dried L edodes contained <1500 spores/kg of proteolytic C. botulinutn and it was not possible to determine reliable detection limits for spores of non-proteolytic C botulinum using the current detection protocol. (C) 2013 Elsevier B.V. All rights reserved.

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