您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Adherence and associated virulence gene expression in acid-treated Escherichia coli O157:H7 in vitro and in ligated pig intestine

文献类型： 外文期刊

作者： Yin, Xianhua 1 ; Feng, Yanni 3 ; Lu, Yang 4 ; Chambers, James R. 2 ; Gong, Joshua 2 ; Gyles, Carlton L. 1 ;

作者机构： 1.Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1, Canada

2.Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada

3.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China

4.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China

期刊名称：MICROBIOLOGY-SGM （ 影响因子：2.777； 五年影响因子：2.871 ）

ISSN： 1350-0872

年卷期： 2012 年 158 卷

页码：

收录情况： SCI

摘要： Escherichia coli O157 : H7 cells that interact with intestinal epithelial cells in animals and humans do so after passage through the low pH of the stomach. This study compared adherence and its associated virulence gene expression in acid-treated (AT) and non-acid treated (NAT) E. coli O157 : H7 strain 86-24 in vitro and in ligated pig intestine. It was found that in vitro, AT O157 : H7 had significantly decreased adherence accompanied by decreased expression of stcE and toxB but not of the locus of enterocyte effacement (LEE) genes. Expression of gadE, genes involved in quorum sensing, and the global regulators cyaA, hfq, Irp, fis and himA was significantly increased; notably, ureD expression was increased 29-fold compared with NAT O157 : H7. AT O157 : H7 colonized the pig intestine as effectively as NAT O157 : H7 bacteria. Expression of 70 of 72 virulence genes from bacteria recovered from the intestine was similar between AT and NAT O157 : H7, except ureD, pagC and bax, whose level of expression was reduced in the AT bacteria. Genes involved in acid response, regulators gadE, cyaA and hfq, and toxin synthesis genes (stx2A and stx2B) were expressed at significantly reduced levels in the intestine by both AT and NAT strains. Expression in the intestine of the LEE and putative adhesion factors cahA, iha and Ipf2 was at levels similar to those in vitro, while ehaA and ureD in NAT O157 : H7 were expressed significantly more highly in vivo than in vitro. These data indicate that AT and NAT O157 : H7 behave differently, and that expression of their virulence genes is regulated differently in vitro from in vivo.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台