Determination of Carbohydrates by High Performance Anion Chromatography-Pulsed Amperometric Detection in Mushrooms
文献类型: 外文期刊
作者: Zhou, Shuai 1 ; Tang, Qing-Jiu 1 ; Luo, Xi 1 ; Xue, Jun-Jie 1 ; Liu, Yan-Fang 1 ; Yang, Yan 1 ; Zhang, Jin-Song 1 ; Feng, 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Key Lab Appl Mycol Resources & Utilizat, Minist Agr, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China; Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China
关键词: medicinal mushrooms;high performance anion chromatography;pulsed amperometric detection;trehalose;mannitol;arabitol;glucose;carbohydrates
期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )
ISSN: 1521-9437
年卷期: 2012 年 14 卷 4 期
页码:
收录情况: SCI
摘要: A method of detecting carbohydrates (fucose, trehalose, mannitol, arabitol, mannose, glucose, galactose, fructose, and ribose) by high-performance anion chromatography pulsed amperometric detection (HAPEC-PAD) was established. The conditions are: CarboPac MA1 column, NaOH as the eluent, temperature 30 degrees C, Au working electrode, Ag/AgCl reference electrode, and flow rate 0.4 mL/min. These nine analytes, which yielded high resolution by this method, could be detected in 40 minutes. Mushrooms were tested and good precision, stability, and reproducibility were achieved. This method is suitable for mushroom samples and could support research and development on sugar and sugar alcohol, which contains special effects.
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