文献类型： 外文期刊

作者： Li, Jinhong ¹ ; Wang, Yiwei ¹ ; Zhao, Yuche ¹ ; Zhang, Shirui ³ ; Wang, Chunyu ¹ ; Cong, Ling ¹ ; Zhang, Yanpeng ¹ ; Gang, Shuang ² ; Lu, Xiaochun ¹ ;

作者机构： 1.Liaoning Acad Agr Sci, Sorghum Res Inst, Shenyang 110161, Peoples R China

2.Shenyang Univ, Sch Life Sci & Engn, Shenyang 110044, Peoples R China

3.Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China

4.Shenyang Univ, Key Lab Ecol Restorat Reg Polluted Environm, Minist Educ, Shenyang 110044, Peoples R China

关键词： Sorghum bicolor; nal6; phenotype identification; transcriptome analysis; DEGs

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Leaf morphology influences photosynthesis, transpiration, and, ultimately, crop yield. To elucidate the molecular regulatory mechanisms underlying narrow leaves in Sorghum bicolor, we identified key DEGs (differentially expressed genes) influencing leaf morphology. The nal6 (the narrow-leaf mutant6) was obtained through 0.1% EMS (ethyl methane sulfonate) chemical mutagenesis of the WT (BTX623). Compared with the WT leaves, there were significant differences in leaf width and length at the flowering stage. A total of 1520 DEGs between the nal6 and WT were screened at the flowering stage based on the transcriptome analysis of sword leaves. KEGG and GO enrichment analyses revealed that DEGs were significantly enriched in pathways such as plant signal transduction, cytokinin biosynthesis, photosynthetic antenna proteins, and secondary metabolite biosynthesis. Further analysis indicated that four DEGs are involved in regulating auxin signaling transduction, thirteen DEGs are involved in regulating zeatin signal transduction, and two DEGs are involved in regulating zeatin biosynthesis. These genes are differentially expressed in nal6, directly affecting the signaling of auxin and zeatin and the biosynthesis of zeatin. Our findings provide a theoretical foundation for understanding the molecular regulation of narrow leaves and breeding ideal plant types in Sorghum bicolor.