A random amplified polymorphic DNA (RAPD) molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp chinensis Makino L.)
文献类型： 外文期刊
作者： Du, Zhengxiang 1 ; Hou, Ruixian 1 ; Zhu, Yuying 1 ; Li, Xiaofeng 1 ; Zhu, Hongfang 1 ; Wang, Zhenzhen 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai Key Lab Protected Hort Technol, Shanghai 201106, Peoples R China
关键词： Pak-choi;late bolting;random amplified polymorphic DNA (RAPD);bulked segregant analysis (BSA)
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 41 期
页码：
收录情况： SCI
摘要： Bulked segregant analysis (BSA) and random amplified polymorphic DNA (RAPD) methods were used to analyze F(2) individuals of P-27 x P-28 to screen and characterize the molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.). A total of 200 random primers were used for RAPD analysis. One RAPD marker S265(750) was identified to be co-segregating with the late-bolting gene, and the genetic distances between S265(750) and late-bolting gene was 3.14 cM. The results of the study can be seen as a starting point for future researches on the pak-choi bolting gene mapping and molecular marker assisted breeding.
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