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Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15

文献类型： 外文期刊

作者： Zhuang, Jing 1 ; Sun, Chao-Cai 1 ; Zhou, Xi-Rong 1 ; Xiong, Ai-Sheng 3 ; Zhang, Jian 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China

2.Alberta Innovates Technol Futures, Life Sci, Vegreville, AB T9C 1T4, Canada

3.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： Transcription factor;AP2 family;BnaRAV-1-HY15;Abiotic stresses;Brassica napus L.;HuYou15

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2011 年 38 卷 6 期

页码：

收录情况： SCI

摘要： Transcriptional regulation is thought to be important for stress tolerance and response of transcription factors. RAV subfamily transcription factor contains an AP2- and B3-DNA binding domain, which belongs to the AP2/ERF family. It encodes transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants. Here, a RAV-like gene, BnaRAV-1-HY15, was isolated from Brassica napus L. cv HuYou15. Sequence homology analysis revealed that the BnaRAV-1-HY15 factor belongs to the RAV subfamily of the AP2/ERF family, and it shares high identity with the AtRAV2 of Arabidopsis. Sequence and three-dimensional structural analyses revealed that BnaRAV-1-HY15 contains two distinct DNA-binding domains, one AP2 domain together with one B3 domain. The AP2 domain composed of 54 amino acids and present in N-terminal region. In addition to AP2 domain, 117 amino acids show significant sequence similarity to the B3 domain present in C-terminal region. Semi-quantitative RT-PCR analysis indicated that the BnaRAV-1-HY15 gene is induced by cold, NaCl and PEG treatments. Under ABA stress, the expression of BnaRAV-1-HY15 gene was not detected. The gene expression was also not traceable from the tissues of pod, bud, petal, leaf, stem and root of normally grown B. napus L. HuYou15 plant at the period of flowering and seed development.

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[1]BREEDING OF WAXLESS TWO-TYPE LINE OF RECESSIVE GENIC MALE STERILITY IN Brassica napus L.. Zhou Xirong,Li Shulin,Zhuang Jing,Gu Longdi. 2001

[2]Breeding and utilization of recessive genic male sterile dual-purpose Line 20118AB in Brassica napus L.. SUN Chaocai,ZHAO Hua,WANG Weirong,LI Yanli,QIAN Xiaofang,FANG Guanghua. 2007

[3]Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza sativa L.. Fu, Xiao-Yan,Zhang, Zhen,Peng, Ri-He,Xiong, Ai-Sheng,Liu, Jin-Ge,Wu, Li-Juan,Gao, Feng,Zhu, Hong,Guo, Zhao-Kui,Yao, Quan-Hong. 2007

[4]Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Zhuang, Jing,Yao, Quan-Hong,Sun, Chao-Cai,Zhou, Xi-Rong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min,Xiong, Fei.

[5]Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.. Liu, Jin-Ge,Zhang, Zhen,Qin, Qiu-Lin,Peng, Ri-He,Xiong, Ai-Sheng,Chen, Jian-Min,Xu, Fang,Zhu, Hong,Yao, Quan-Hong.

[6]Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution. Jin, Xiao-Fen,Zhu, Bo,Peng, Ri-He,Jiang, Hai-hua,Yao, Quan-Hong,Xiong, Ai-Sheng,Jiang, Hai-hua,Chen, Jian-Min,Zhuang, Jing,Zhang, Jian.

[7]Transcription factor OsAP21 gene increases salt/drought tolerance in transgenic Arabidopsis thaliana. Jin, Xiaofeng,Xue, Yong,Xu, RanRan,Bian, Lin,Zhu, Bo,Han, Hongjuan,Peng, Rihe,Yao, Quanhong,Wang, Ren.

[8]Genome-Wide Identification and Analysis of the MYB Transcription Factor Superfamily in Solanum lycopersicum. Li, Zhenjun,Peng, Rihe,Tian, Yongsheng,Han, Hongjuan,Xu, Jing,Yao, Quanhong.

[9]A novel Cys(2)/His(2) zinc finger protein gene from sweetpotato, IbZFP1, is involved in salt and drought tolerance in transgenic Arabidopsis. Wang, Feibing,Peng, Rihe,Yao, Quanhong,Zhu, Hong,Liu, Qingchang,Tong, Wenjie,Kong, Weili.

[10]Discovery, phylogeny and expression patterns of AP2-like genes in maize. Zhuang, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Deng, De-Xiang,Xiong, Fei,Chen, Jian-Min,Zhuang, Jing,Zhang, Jian.

[11]Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis. Xiong, Ai-Sheng,Wang, Feng,Jiang, Hai-Hua,Peng, Ri-He,Jin, Xiao-Fen,Zhu, Bo,Yao, Quan-Hong,Zhuang, Jing,Zhang, Jian.

[12]Analysis of Brassica rapa ESTs: gene discovery and expression patterns of AP2/ERF family genes. Zhuang, Jing,Xiong, Ai-Sheng,Peng, Ri-He,Gao, Feng,Zhu, Bo,Fu, Xiao-Yan,Jin, Xiao-Feng,Yao, Quan-Hong,Zhang, Zhen,Qiao, Yu-Shan,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min.

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