您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3

文献类型： 外文期刊

作者： Ji, Zhe 1 ; Tang, Qingju 1 ; Hao, Ruixa 1 ; Zhang, Jinsong 1 ; Pan, Yingje 3 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai Key Lab Agr Genet & Breeding,Minist Agr, Natl Engn Res Ctr Edible Fungi,Key Lab Appl Mycol, Shanghai 201106, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab, Inst Nutr Sci, Shanghai 200031, Peoples R China

3.Shanghai Fisheries Univ, Shanghai 200090, Peoples R China

关键词： apoptosis;Ganoderma lucidum;triterpene

期刊名称：ONCOLOGY LETTERS （ 影响因子：2.967； 五年影响因子：2.575 ）

ISSN： 1792-1074

年卷期： 2011 年 2 卷 3 期

页码：

收录情况： SCI

摘要： The medicinal mushroom Ganoderma lucidum (G. lucidum) has been used for the treatment of various diseases, and is known for the immune-enhancing activity of its polysaccharide. However, little is known about another of its major constituents, triterpene. This study investigated the anticancer mechanism of a triterpene-enriched extract from G. lucidum. The triterpene-enriched extract, GLAI, was prepared from fruiting bodies of G. lucidum by sequential hot water extraction, removal of ethanol-insoluble polysaccharides and gel-filtration chromatography. The mechanisms of GLAI-induced apoptosis on SW620 human colorectal adenocarcinoma cells were investigated. Tumor cell lines in vitro were treated with different concentrations of GLAI. Cell proliferation was measured by the Alamar blue assay, morphology of cell apoptosis was observed, cell apoptosis was detected by flow cytometry (FCM) and caspase-3 activity was detected by Caspase-3 cellular activity assay. The results showed that GLAI inhibited the growth of different tumor cells and caused significant apoptosis in a dose-dependent manner. Marked morphological changes of cell apoptosis were observed after the cells had been exposed to GLAI for 24 h. The Caspase-3 assay results showed that the activity of the caspase-3 enzyme increased in both a time- and dose-dependent manner, whereas GLAI resulted in the down-regulation of Bcl-2 gene expression at the mRNA level and XIAP protein production at the protein level. Conversely, GLAI up-regulates the expression of the apoptosis enhancer Bax gene and p53 protein. These findings suggest that the triterpenes contained in G. lucidum are potential anticancer agents.

  • 相关文献

[1]An Unstructured Kinetic Model for the Improvement of Triterpenes Production by Ganoderma lucidum G0119 Based on Nitrogen Source Effect. Feng, Jie,Zhang, Jing-Song,Jia, Wei,Yang, Yan,Liu, Fang,Lin, Chi-Chung.

[2]Production of IgM by B Cells After Stimulation with GLIS from Ganoderma lucidum Mushroom. QINGJIU TANG,JINGSONG ZHANG,WERNER REUTTER,HUA FAN,Shanghai Academy of Agricultural Sciences(SAAS); Shanghai Academy of Agricultural Sciences(SAAS);. 2005

[3]One single standard substance for the simultaneous determination of 17 triterpenes in Ganoderma lingzhi and its related species using high-performance liquid chromatography. Liu, Wei,Zhang, Jingsong,Liu, Yanfang,Feng, Jie,Tang, Chuanhong,Feng, Na,Tang, Qingjiu,Liu, Wei,Han, Wei. 2017

[4]Purification, NMR Study and Immunostimulating Property of a Fucogalactan from the Fruiting Bodies of Ganoderma lucidum. Ye, Libin,Zhang, JingSong,Yang, Yan,Zhou, Shuai,Jia, Wei,Hao, RuiXia,Ye, Libin,Zhou, Kan,Pan, Yinglie. 2008

[5]Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers. Chao Wen-zheng,Yu Ling,Tang Chuan-hong,Zhang Jing-song,Tang Chuan-hong,Honda, Yoichi. 2018

[6]Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes). Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi. 2013

[7]A novel Ganoderma lucidum G0119 fermentation strategy for enhanced triterpenes production by statistical process optimization and addition of oleic acid. Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Zhang, Jing-Song,Feng, Na,Yan, Meng-Qiu,Yang, Yan,Jia, Wei,Lin, Chi-Chung.

[8]Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum. Shi, Liang,Qin, Lei,Ren, Ang,Fang, Xing,Mu, Dashuai,Zhao, Mingwen,Xu, Yingjie,Tan, Qi.

[9]Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies. Ye, LiBin,Zhang, JingSong,Tang, QingJiu,Liu, YanFang,Ye, LiBin,Du, XiuJui,Ye, XiJun,Gong, ChunYu,Pan, YingJie.

[10]Structural characterization and immuno-enhancing activity of a highly branched water-soluble beta-glucan from the spores of Ganoderma lucidum. Wang, Yatao,Liu, Yanfang,Zhou, Shuai,Zhang, Zhong,Wu, Di,Yan, Mengqiu,Tang, Qingjiu,Zhang, Jingsong,Wang, Yatao,Yan, Mengqiu,Yu, Huazheng.

[11]Preparation of a novel sulfated glycopeptide complex and inhibiting L1210 cell lines property in vitro. Ye, LiBin,Zhang, JingSong,Zhou, Shuai,Ye, LiBin,Wang, Sheng,Wu, Di,Pan, YingJie.

[12]Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum. Zhao, Ming-Wen,Liang, Wan-Qi,Zhang, Da-Bing,Wang, Nan,Wang, Chen-Guang,Pan, Ying-Jie. 2007

[13]NMR characterization for polysaccharide moiety of a glycopeptide. Zhang, Jingsong,Ye, Libin,Li, Jianrong,Pan, Yingjie. 2010

[14]Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum. Shi, Liang,Fang, Xing,Li, Mengjiao,Mu, Dashuai,Ren, Ang,Zhao, Mingwen,Tan, Qi. 2012

[15]Immunomodulation of Bone Marrow Macrophages by GLIS, a Proteoglycan Fraction from Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidium (W.Curt.: Fr.) P. Karst.. Ji, Zhe,Tang, Qingjiu,Zhang, Jinsong,Yang, Yan,Liu, Yanfang,Ji, Zhe,Pan, Yingjie. 2011

[16]Physicochemical characterization of a high molecular weight bioactive beta-D-glucan from the fruiting bodies of Ganoderma lucidum. Liu, Yanfang,Zhang, Jingsong,Tang, Qingjiu,Yang, Yan,Wu, Di,Guo, Qingbin,Wang, Qi,Cui, Steve W.. 2014

[17]Biochemical characterization of a proteoglycan complex from an edible mushroom Ganoderma lucidum fruiting bodies and its immunoregulatory activity. Ye, LiBin,Zheng, Xiaolin,Wang, Xiangyang,Li, Jianrong,Zhang, Jingsong,Tang, Qingqiu,Yang, Yan,Liu, YanFang,Pan, YingJie.

[18]A New Temperature Control Shifting Strategy for Enhanced Triterpene Production by Ganoderma lucidum G0119 Based on Submerged Liquid Fermentation. Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung,Feng, Jie,Feng, Na,Zhang, Jing-Song,Yang, Yan,Jia, Wei,Lin, Chi-Chung.

[19]Structural Characterisation Of A Heteropolysaccharide By Nmr Spectra. Ye, LiBin,Zhang, JingSong,Yang, Yan,Zhou, Shuai,Liu, YanFang,Tang, Qinglui,Du, Xiuju,Chen, Hui,Ye, LiBin,Du, Xiuju,Pan, YingJie.

[20]Simple and Reproducible Two-Stage Agitation Speed Control Strategy for Enhanced Triterpene Production by Lingzhi or Reishi Medicinal Mushrooms, Ganoderma lucidum ACCC G0119 (Higher Basidiomycetes) Based on Submerged Liquid Fermentation. Feng, Jie,Feng, Na,Yang, Yan,Liu, Fang,Zhang, Jing-Song,Jia, Wei,Feng, Jie,Feng, Na,Yang, Yan,Liu, Fang,Zhang, Jing-Song,Jia, Wei,Lin, Chi-Chung.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台