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A Sequence-amplified Characterized Region Marker for a Single, Dominant Gene in Melon PI 134198 that Confers Resistance to a Unique Race of Podosphaera xanthii in China

文献类型： 外文期刊

作者： Liu, Longzhou 1 ; Chen, Youyuan 1 ; Su, Zhenghong 1 ; Zhang, Hui 1 ; Zhu, Weiming 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai Key Lab Protected Hort, Shanghai 201106, Peoples R China

关键词： Cucumis melo;marker assisted selection;SRAP marker;bulk segregant analysis

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

ISSN： 0018-5345

年卷期： 2010 年 45 卷 9 期

页码：

收录情况： SCI

摘要： Melon production in China is threatened by cucurbit powdery mildew incited by Podosphaera xanthii, which can cause severe yield losses. Disease reactions of 10 melon P. xanthii race differentials indicated the presence of a unique race of P. xanthii, denoted pxCh1, in the Shanghai area. Resistance to P. xanthii race pxCh1 in melon PI 134198, which originated from India, was controlled by a single, dominant gene, designated Pm-8, in a cross with susceptible melon inbred S717. A search for a molecular marker linked to Pm-8 was initiated using the same cross. Bulk segregant analysis of their F(2) progeny revealed one sequence-related amplified polymorphism (SRAP) marker of 148 bp linked to Pm-8, at 3.9 cM. The SRAP segment was recovered, cloned, and sequenced and converted into a sequence-amplified characterized region marker for marker-assisted selection of Pm-8 in breeding programs to develop melon cultivars resistant to P. xanthii race pxCh1.

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