文献类型： 外文期刊

作者： Ai, L. ¹ ; Dong, S. J. ² ; Zhang, W. Y. ³ ; Elsheikha, H. M. ⁴ ; Mahmmod, Y. S. ¹ ; Lin, R. Q. ¹ ; Yuan, Z. G. ¹ ; Shi, Y ¹ ;

作者机构： 1.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

2.Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai 201106, Peoples R China

3.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Guangxi Zhuang, Peoples R China

4.Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics, England

5.Zagazig Univ, Fac Vet Med, Anim Med Dept, Zagazig 44511, Sharkia Prov, Egypt

6.CAAS, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Aetiol Biol, Lanzhou 730046, Gansu, Peoples R China

期刊名称：ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY （ 影响因子：1.203； 五年影响因子：1.453 ）

ISSN： 0003-4983

年卷期： 2010 年 104 卷 1 期

页码：

收录情况： SCI

摘要： Among the helminths infecting ruminants in China are three taxa belonging to the genus Fasciola: F. hepatica, F. gigantica and the so-called 'intermediate form' that appears to lie between these two species. Based on the sequences of the second internal-transcribed spacers (ITS-2) within the parasites' nuclear ribosomal DNA (rDNA), a pair of primers (DSJf/DSJ3) specific for F. hepatica and a pair (DSJf/DSJ4) specific for F. gigantica were designed and used to develop PCR-based assays. These assays allowed the identification and differentiation of F. hepatica, F. gigantica and the 'intermediate' Fasciola, with no amplicons produced from heterologous DNA samples. The results of sequencing confirmed the species-specific identity of the amplified products. The assays showed good sensitivity, giving positive results with as little as 0.11 ng of F. hepatica DNA and 0.35 ng of F. gigantica DNA. This meant that the DNA from a single Fasciola egg or a single infected snail was sufficient for identification of the Fasciola taxon. The developed PCR assays could provide useful tools for the detection, identification and epidemiological investigation of Fasciola infection in humans, other mammals and snails.