文献类型： 外文期刊

作者： Lin, R. Q. ¹ ; Dong, S. J. ² ; Nie, K. ³ ; Wang, C. R.; Song, H. Q.; Li, A. X.; Huang, W. Y.; Zhu, X. Q.;

作者机构： 1.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

2.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China; Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai 201106, Peoples R China; Southwest Univ, Coll Anim Sci & Technol, Chongqing 400715, Peoples R China; Heilongjiang August Firest Land Reclamat Univ, Coll Anim Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China; Zhongshan Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China

3.S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China; Shanghai Acad Agr Sci, Anim Husb & Vet Res Inst, Shanghai 201106, Peoples R China; Southwest Univ, Coll Anim Sci & Technol, Chongqing 400715, Peoples R China; Heilongjiang August Firest Land Reclamat Univ, Coll Anim Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China; Zhongshan Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Guangxi Univ

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2007 年 101 卷 3 期

页码：

收录情况： SCI

摘要： In the present study, a polymerase chain reaction-linked single-strand conformation polymorphism (PCR-SSCP) approach combined with DNA sequencing was used to characterise samples of Fasciola spp. from different host species and geographical locations in mainland China. The first internal transcribed spacer (ITS-1) of ribosomal DNA (rDNA) was amplified by PCR from individual Fasciola and analysed by SSCP. SSCP analyses displayed three different banding profiles that allowed the identification of all Fasciola samples examined into three groups: Fasciola hepatica, F. gigantica and the "intermediate" Fasciola. Then, the ITS-1 rDNA was sequenced from representative Fasciola samples, and analysis of the complete ITS-1 sequences supported the identification of all Fasciola samples by SSCP approach. The length of the ITS-1 sequences was 422 bp for all Fasciola samples sequenced. Although there was no variation in length or composition of the ITS-1 sequences among multiple specimens within each of the taxa, F. hepatica and F. gigantica differed by 1.2% in their ITS-1 sequences, whereas the "intermediate" Fasciola was unique, in which two different ITS-1 sequences exist in the rDNA array within a single Fasciola worm. One of the sequences is identical to that of F. hepatica, and the other is identical to that of F. gigantica. This study demonstrated that PCR-SSCP analysis of the ITS-1 rDNA followed by selective sequencing provides a reliable approach for the accurate identification of Fasciola spp., and also supports the existence of the "intermediate" Fasciola between F. hepatica and F. gigantica in mainland China.