Integrated Transcriptomic Analysis of Liver and Muscle Tissues Reveals Candidate Genes and Pathways Regulating Intramuscular Fat Deposition in Beef Cattle
文献类型： 外文期刊
作者： Wang, Siwei 1 ; Liu, Tingting 2 ; Peng, Peng 2 ; Fu, Yurong 2 ; Shi, Shaoqing 2 ; Liang, Shuang 2 ; Chen, Xi 2 ; Wang, Kun 2 ; Zhou, Rongyan 1 ;
作者机构： 1.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Key Lab Crop Cultivat Physiol & Green Prod Hebei P, Shijiazhuang 050035, Peoples R China
关键词：
intramuscular fat; beef cattle; transcriptomic;
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2025 年 15 卷 9 期
页码：
收录情况： SCI
摘要： Intramuscular fat (IMF) content in beef cattle is a critical determinant of beef meat quality, as it positively influences juiciness, tenderness, and palatability. In China, the crossbreeding of Wagyu and Angus is a prevalent method for achieving a better marbling level. However, the molecular mechanisms governing IMF regulation in these crossbreeds remain poorly understood. To elucidate the mechanism of IMF deposition in these crossbred cattle, we conducted a comparative transcriptomic analysis of longissimus dorsi muscles and livers from cattle with divergent IMF content. RNA-seq revealed 940 and 429 differentially expressed genes (DEGs) in the liver and muscle, respectively, with 60 genes co-differentially expressed (co-DEGs) in both tissues. Functional enrichment highlighted lipid metabolism pathways including fatty acid beta-oxidation, PPAR signaling, and glycerolipid metabolism. A total of eleven genes including ACAA2, ACADL, ACOX2, CPT1B, CPT2, LPL, SLC27A1, ACAT1, GK, ACOX3, and ACSM5, were screened as key candidate genes for IMF deposition. A "liver-muscle" regulatory network of IMF deposition was built to illustrate the tissues' interaction. The reliability of the transcriptomic data was verified by quantitative reverse real-time PCR (qRT-PCR). Our findings provide novel molecular markers for increasing the IMF content and accelerating the genetic improvement of beef quality traits in crossbred cattle.
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