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The H protein of attenuated canine distemper virus is degraded via endoplasmic reticulum-associated protein degradation

文献类型： 外文期刊

作者： Wang, Wenjie 1 ; Bi, Zhenwei 2 ; Liu, Yakun 3 ; Xia, Xingxia 2 ; Qian, Jing 2 ; Tan, Yeping 2 ; Zhao, Jianjun 3 ; Song, Suquan 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol,Minist Agr & Rural, Nanjing, Jiangsu, Peoples R China

3.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing, Heilongjiang, Peoples R China

关键词： canine distemper virus; H protein; degradation; ERAD; replication

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： Canine distemper (CD) caused by canine distemper virus (CDV) is considered a highly contagious and acutely febrile disease in various animals around the world. Endoplasmic reticulum-associated protein degradation (ERAD) is an important biological effect induced by endoplasmic reticulum (ER) stress (ERS) for the degradation of unfolded/misfolded proteins in the ER of cells. CDV H glycoprotein is translocated into the ER for post-translational modifications. The effects of CDV H and ER on each other are unclear. In this study, we found that CDV H protein induced ERS through the PERK-mediated signaling pathway. The inhibition of ERS by 4-Phenylbutyric acid (4-PBA) increased the H protein amounts of an attenuated CDV, which was reduced by dithiothreitol (DTT)-induced ERS. Further, the H protein levels were increased when ERAD was inhibited by using Eeyarestatin I or interfering E3 ligase Hrd1 in ERAD, suggesting that the attenuated CDV H protein is degraded via ERAD. ERAD involved ubiquitin-dependent proteasome degradation (UPD) and/or autophagic-lysosome degradation (ALD). The attenuated CDV H protein was ubiquitinated and significantly increased after treatment with UPD inhibitor MG132 but not ALD inhibitor chloroquine (CQ), suggesting that ERAD degrading the attenuated CDV H protein selectively depends on UPD. Moreover, the inhibition of the degradation of CDV H protein with 4-PBA or MG132 treatment increased viral replication, whereas treatment with DTT promoting degradation of H protein was found to reduce viral replication. These findings suggest that the degradation of CDV H protein via ERAD negatively affects viral replication and provide a new idea for developing CDV prevention and control strategies.

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