Determination of 18 beta-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry
文献类型： 外文期刊
作者： Han, Feng 1 ; Chen, Qing-ping 1 ; Yang, Guangxin 1 ; Zhai, Wenlei 2 ; Fodjo, Essy Kouadio 3 ; Tian, Liang-liang 1 ; Yu, Hui-juan 1 ; Shi, Yong-fu 1 ; Kong, Cong 1 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
3.Univ Felix Houphouet Boigny, Phys Chem Lab, Abidjan, Cote Ivoire
关键词： beta-agonist; ractopamine; aquatic product; enzymolysis; fish; veterinary drug; banned drug
期刊名称：ACS FOOD SCIENCE & TECHNOLOGY （ 影响因子：2.3； 五年影响因子：2.3 ）
ISSN：
年卷期： 2022 年 2 卷 2 期
页码：
收录情况： SCI
摘要： An effective and efficient method for the determination of 18 beta-agonists in aquatic products using liquid chromatography tandem mass spectrometry (LC-MS/MS) was successfully established. The method optimization was conducted based on incurred samples to guarantee its practicability. In this method, the sample was hydrolyzed with beta-glucuronidase-arylsulfatase in ammonium acetate solution and treated successively with protein precipitation in 5% trichloroacetic acid in water, lipid removal with n-hexane, and further concentration and cleanup by solid-phase extraction (hydrophilic-lipophilic balanced cartridge, primary-secondary amine cartridge). Isotope-labeled internal standards and matrix-matched calibration curves were used for quantification. The limit of quantification (LOQ) was verified at 0.5-1.0 mu g/kg (S/N > 10). This method was evaluated with five different matrices, including eel, roasted eel, pufferfish, shrimp, and crab, at 1x, 2x, and 10x LOQs, with good recoveries (>77%) and high replicate repeatability (relative standard deviations (RSDs) <13%, intraday batch repeatability) and further validated by three other accredited laboratories. A total of 3 samples out of 37 tested aquatic products were found positive for unknown incur sources (ractopamine, 0.38-2.1 mu g/kg; salbutamol 3.0 mu g/kg; tulobuterol 2.9 mu g/kg; clenbuterol 1.3 mu g/kg; and clenpenterol, 0.3 mu g/kg). The method has demonstrated to be reliable, sensitive, and stable for multiresidue screening of beta-agonists for food safety monitoring.
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