Single-cell RNA-seq reveals the effects of the FecB mutation on the transcriptome profile in ovine cumulus cells
文献类型： 外文期刊
作者： Guo, Xiaofei 1 ; Fang, Yi 4 ; Liang, Rong 4 ; Wang, Xiangyu 3 ; Zhang, Jinlong 2 ; Dong, Chunxiao 2 ; Wang, Biao 2 ; Liu, Yu 2 ; Chu, Mingxing 3 ; Zhang, Xiaoshen 2 ; Zhong, Rongzhen 1 ;
作者机构： 1.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Jilin Prov Feed Proc & Ruminant Precis Breeding Cr, Jilin Prov Lab Grassland Farming,State Key Lab Bla, Changchun 130102, Peoples R China
2.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China
3.Chinese Acad Agr Sci CAAS, Inst Anim Sci, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China
4.Jilin Agr Univ, Key Lab Anim Prod Prod Qual & Secur, Minist Educ, Changchun 130118, Peoples R China
关键词： Single-cell RNA-seq; Cumulus cells; Follicle; FecB; Sheep
期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.8； 五年影响因子：4.3 ）
ISSN： 2045-2322
年卷期： 2024 年 14 卷 1 期
页码：
收录情况： SCI
摘要： Genetic variations in the ovine ovulation rate, which are associated with the FecB mutation, provide useful models by which to explore the mechanisms regulating the development of mammalian antral follicles. In order to study the effects of the FecB mutation on cumulus cell differentiation, preovulatory follicles were aspirated and cumulus cells were isolated from three FecB genotypes (homozygous, heterozygous and wild type) of Small Tail Han (STH) sheep superstimulated with FSH. Transcriptome information from tens of thousands of cumulus cells was determined with the 10 x Genomics single-cell RNA-seq technology. Under the superovulation treatment, the observed number of preovulatory follicles in the ovaries of FecB carriers was still significantly higher than that in the wild-type (P < 0.05). The expression patterns of cumulus cells differed between FecB carriers and wild-type ewes. The screened cumulus cells could also be further divided into different cell clusters, and the differentiation states and fates of each group of cumulus cells also remained different, which supports the notion that heterogeneity in gene expression is prevalent in single cells. The oxidative phosphorylation pathway was significantly enriched in differentially expressed genes among the cell differentiation branch nodes of cumulus cells and among the differentially expressed genes of cumulus cells from the three genotypes. Combined with the important role of oxidative phosphorylation in the maturation of COCs, we suggest that the oxidative phosphorylation pathway of cumulus cells plays a crucial role in the differentiation process of cumulus cells and the mutation effect of the FecB gene.
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