Production of active human FGF21 using tobacco mosaic virus-based transient expression system
文献类型： 外文期刊
作者： Fan, Jieying 1 ; Wang, Yunpeng 1 ; Huang, Shuang 1 ; Xing, Shaochen 1 ; Wei, Zhengyi 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun, Peoples R China
关键词： Transient expression; tobacco mosaic virus; human FGF21; Agrobacterium-mediated vacuum infiltration
期刊名称：GROWTH FACTORS （ 影响因子：2.394； 五年影响因子：2.765 ）
ISSN： 0897-7194
年卷期： 2022 年 39 卷 1-6 期
页码：
收录情况： SCI
摘要： Fibroblast growth factor (FGF) family has a wide range of metabolic processes. FGF21 exerts critical physiological functions in clinical application. This study aimed to explore a convenient and highly efficient approach for rhFGF21 expression using TMV-TES. Firstly, the vector pTTEV-GFP was constructed, followed by optimisation of the expression parameters in Nicotiana benthamiana. Then, the rhFGF21 encoding gene harbouring vector pTTEV-rhFGF21 was constructed. Agrobacterium-mediated vacuum infiltration was performed with the optimised parameters and the expression of rhFGF21 was confirmed by the immunoblotting analysis. ELISA revealed that the protein accumulation of rhFGF21 accounts for 0.11% of total soluble proteins. The biological activity was evaluated and the results suggested that tobacco-expressed rhFGF21 could stimulate the glucose uptake in swiss 3T3-L1 adipocytes, which was similar to the activity of commercial products, suggesting its native biological activity. Therefore, using TMV-TES to express rhFGF21 will be a feasible approach for the mass production of rhFGF21.
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