文献类型： 外文期刊

作者： Jin, Jing ¹ ; Gan, Kexin ² ; Zhao, Lan ¹ ; Jia, Huijuan ² ; Zhu, Yifan ² ; Li, Xiongwei ³ ; Yang, Zhaowei ⁴ ; Ye, Zhengwe ¹ ;

作者机构： 1.Zhejiang Univ, Allergy Res Ctr, Hangzhou, Peoples R China

2.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou, Peoples R China

3.Shanghai Acad Agr Sci, Forest & Fruit Tree Inst, Shanghai, Peoples R China

4.Guangzhou Med Univ, State Key Lab Resp Dis, Affiliated Hosp 1, Guangzhou, Peoples R China

5.China Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou, Peoples R China

6.Jiangsu Acad Agr Sci, Fruit Tree Inst, Nanjing, Peoples R China

7.Hebei Acad Agr & Forestry Sci, Shijiazhuang Pomol Inst, Shijiazhuang, Hebei, Peoples R China

8.Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China

9.CSIC IRTA UAB UB, IRTA, Edifici CRAG, Campus UAB, Barcelona, Spain

10.Univ Amsterdam, Amsterdam UMC, Dept Expt Immunol, Amsterdam, Netherlands

11.Univ Amsterdam, Amsterdam UMC, Dept Otorhinolaryngol, Amsterdam, Netherlands

关键词： ELISA; hypoallergenic varieties; peach allergy; protein quantification; Pru p 1; 酶 联 免 疫 吸 附 测 试 低 致 敏 品 种 桃 过 敏 蛋 白 定 量 Pru p 1

期刊名称：CLINICAL AND TRANSLATIONAL ALLERGY （ 影响因子：5.871； 五年影响因子：4.891 ）

ISSN：

年卷期： 2021 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Background Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20-73.44 mu g/g, fresh weight), being very low in the pulp (0.05-9.62 mu g/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 mu g/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late-ripening yellow flesh varieties were usually highly allergenic. Conclusion Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, "Zi Xue Tao," "Wu Yue Xian," and "May Fire," were identified, which could be useful in trials for peach allergy patients.