文献类型： 外文期刊
作者： Sheng, Yong 1 ; Wang, Hengyu 1 ; Ou, Yixin 1 ; Wu, Yingying 1 ; Ding, Wei 1 ; Tao, Meifeng 1 ; Lin, Shuangjun 1 ; Deng, Zixin 1 ; Bai, Linquan 1 ; Kang, Qianjin 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, Joint Int Res Lab Metabol & Dev Sci, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, Shanghai 200240, Peoples R China
3.Haihe Lab Synthet Biol, Tianjin 300308, Peoples R China
4.Shanghai Acad Agr Sci, Inst Edible Fungi, Natl Engn Res Ctr Edible Fungi, Key Lab Appl Mycol Resources & Utilizat South,Min, Shanghai 201403, Peoples R China
期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）
ISSN：
年卷期： 2023 年 14 卷 1 期
页码：
收录情况： SCI
摘要： CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified multiple natural transpositions of insertion sequences (ISs) into cas genes, thus inactivating CRISPR-Cas defenses. We then generated an IS-trapping system, using Escherichia coli strains with various ISs and an inducible cas nuclease, to monitor IS insertions into cas genes following the induction of double-strand DNA breakage as a physiological host stress. We identified multiple events mediated by different ISs, especially IS1 and IS10, displaying substantial relaxed target specificity. IS transposition into cas was maintained in the presence of DNA repair machinery, and transposition into other host defense systems was also detected. Our findings highlight the potential of ISs to counter CRISPR activity, thus increasing bacterial susceptibility to foreign DNA invasion. CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of mobile genetic elements. Here, the authors show that insertion sequences can efficiently insert into cas genes, thus inactivating CRISPR defenses and increasing bacterial susceptibility to foreign DNA invasion.
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