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The host protein CCT8 interacts with PB2 protein of H9N2 AIV and facilitates virus replication

文献类型： 外文期刊

作者： Cai, Yiqin 1 ; Liu, Ye 1 ; Yin, Guihu 1 ; Hu, Jianing 1 ; Gao, Zichen 1 ; Guo, Xinyu 1 ; Wang, Ruiying 1 ; Zhong, Meng 1 ; Liu, Qingtao 1 ; Feng, Xiuli 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Microbiol Chinas, Minist Agr, Nanjing, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing, Peoples R China

关键词： PB2 protein; mass spectrometry; host protein; chaperonin containing TCP-1 subunit 8 (CCT8); viral replication

期刊名称：AVIAN PATHOLOGY （ 影响因子：2.2； 五年影响因子：2.9 ）

ISSN： 0307-9457

年卷期： 2025 年 54 卷 4 期

页码：

收录情况： SCI

摘要： In recent years, the poultry industry and public health safety have been seriously threatened by low pathogenic avian influenza virus (AIV), such as H9N2. PB2, as a member of RNA polymerase complex of avian influenza virus, plays a crucial role in viral replication and the infection process. To elucidate the mechanism of H9N2 influenza virus infection in host cells, PB2 protein was used as target protein to screen the interacting host proteins through CO-IP, silver staining, and protein mass spectrometry. It was identified that chaperonin containing TCP-1 subunit 8 (CCT8) might interact with PB2 protein during viral replication. H9N2 subtype AIV infection induces the upregulation of endogenous CCT8 gene mRNA and protein levels in cells. Knockdown of CCT8 using siRNA resulted in decreased expression at both the protein and mRNA levels for viral PB2 and NS1 within infected cells, leading to a corresponding reduction in progeny virus production. Conversely, the overexpression of CCT8 increased the protein expression of PB2 and NS1, and also enhanced the mRNA levels of PB2 and NS1 genes. Furthermore, the production of progeny viruses was accordingly increased upon overexpression of CCT8 in 293 T cells. These findings highlight the crucial role played by CCT8 in viral replication, providing novel insights for developing strategies to prevent and treat AIV infection.

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