Positional effects of double-stranded RNAs targeting ss-Actin gene affect RNA interference efficiency in Colorado potato beetle
文献类型： 外文期刊
作者： He, Wanwan 1 ; Xu, Wenbo 1 ; Fu, Kaiyun 3 ; Guo, Wenchao 4 ; Kim, Dae Sung 1 ; Zhang, Jiang 1 ;
作者机构： 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China
2.Wuhan Acad Agr Sci, Wuhan 430070, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi 830000, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Microbial Applicat, Urumqi 830000, Peoples R China
关键词： Colorado potato beetle; RNAi; dsRNA; Positional effects; Foliar application; Potato
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）
ISSN： 0048-3575
年卷期： 2022 年 184 卷
页码：
收录情况： SCI
摘要： Pesticide resistance in pests drives the development of RNA interference (RNAi)-based technology as a novel approach for pest control. To investigate the effects of the positional dependency of double-stranded RNAs (dsRNAs), we newly designed four different 200 bp dsRNAs targeting Colorado potato beetle (CPB) ss-Actin gene, termed as dsACT200-1 to dsACT200-4, to compare their insecticidal activity to CPB larvae together with our previously used 200 bp and 700 bp dsRNAs (dsACT200 and dsACT700), respectively (He et al., 2020a). Each of dsRNAs harbors different numbers of expected siRNAs predicted by sequence-based prediction platform, dsACT200 and dsACT200-2 have a relatively higher number of siRNA than other 200 bps dsRNAs. When CPB larvae were fed with in vitro synthesized dsRNA-painted potato leaves, all the tested dsRNAs showed significant effects to protect against CPB larvae. Combined with the survival rate of CPB larvae, ss-Actin gene expression level and the surviving CPB larvae weight, various positional dsRNAs from the same allele showed different plant protection activity against CPB larvae and partially correlated with the predicted siRNA numbers and distribution on the target sequence. This study suggests the specific allelic locus for rational dsRNA design triggering RNAi efficiency for target gene silencing is an essential factor in enhancing the insecticidal activity.
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