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Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

文献类型： 外文期刊

作者： Li, Min 1 ; Jelocnik, Martina 2 ; Yang, Feng 1 ; Gong, Jianseng 3 ; Kaltenboeck, Bernhard 4 ; Polkinghorne, Adam 2 ; F 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China

2.Univ Sunshine Coast, Ctr Anim Hlth Innovat, Fac Sci Hlth Educ & Engn, Maroochydore, Qld, Australia

3.Chinese Acad Agr Sci, Poultry Inst, Yangzhou, Jiangsu, Peoples R China

4.Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA

5.Natl Ctr Engn Res Vet Bioprod, Inst Vet Med, Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol,Minist Agr, Nanjing, Jiangsu, Peopl

关键词： Chlamydia suis;Pig;FRET-PCR;ompA;MLST;Tanglegram

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2017 年 13 卷

页码：

收录情况： SCI

摘要： Background: Chlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study. Results: We report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3% of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed. Conclusions: Genetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spillover' and 'spill-back' of infections to wild animals and humans.

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