您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

文献类型： 外文期刊

作者： Li, Min 1 ; Jelocnik, Martina 2 ; Yang, Feng 1 ; Gong, Jianseng 3 ; Kaltenboeck, Bernhard 4 ; Polkinghorne, Adam 2 ; F 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China

2.Univ Sunshine Coast, Ctr Anim Hlth Innovat, Fac Sci Hlth Educ & Engn, Maroochydore, Qld, Australia

3.Chinese Acad Agr Sci, Poultry Inst, Yangzhou, Jiangsu, Peoples R China

4.Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA

5.Natl Ctr Engn Res Vet Bioprod, Inst Vet Med, Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol,Minist Agr, Nanjing, Jiangsu, Peopl

关键词： Chlamydia suis;Pig;FRET-PCR;ompA;MLST;Tanglegram

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2017 年 13 卷

页码：

收录情况： SCI

摘要： Background: Chlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study. Results: We report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3% of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed. Conclusions: Genetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spillover' and 'spill-back' of infections to wild animals and humans.

  • 相关文献

[1]上海市食源性单核细胞增生李斯特菌MLST分析及毒力基因分布. 成梦雅,王文凯,史贤明,施春雷. 2019

[2]沙门氏菌血清型快速PCR鉴定方法的建立. 方婷子,史贤明,施春雷. 2017

[3]Chinese border disease virus strain JSLS12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus C strain vaccination. Mao, Li,Li, Wenliang,Liu, Xia,Hao, Fei,Yang, Leilei,Deng, Jiawu,Zhang, Wenwen,Jiang, Jieyuan,Mao, Li,Li, Wenliang,Liu, Xia,Hao, Fei,Yang, Leilei,Deng, Jiawu,Zhang, Wenwen,Jiang, Jieyuan,Mao, Li,Li, Wenliang,Liu, Xia,Hao, Fei,Yang, Leilei,Deng, Jiawu,Zhang, Wenwen,Jiang, Jieyuan,Liu, Xia,Deng, Jiawu,Wei, Jianzhong.

[4]Breed-linked polymorphisms of porcine toll-like receptor 2 (TLR2) and TLR4 and the primary investigation on their relationship with prevention against Mycoplasma pneumoniae and bacterial LPS challenge. Fang, Xiaomin,Liu, Xiao,Meng, Cui,Fu, Yanfeng,Wang, Xuemin,Li, Bixia,Tu, Feng,Zhao, Fang,Ren, Shouwen.

[5]Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs. Xiong, Qiyan,Wei, Yanna,Feng, Zhixin,Gan, Yuan,Liu, Zhanjun,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[6]CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae. Fang, Xiaomin,Zhao, Weimin,Xu, Jie,Tu, Feng,Wang, Xuemin,Li, Bixia,Fu, Yanfeng,Ren, Shouwen.

[7]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[8]Long form leptin receptor and SNP effect on reproductive traits during embryo attachment in Suzhong sows. Fu, Yanfeng,Li, Bixia,Fang, Xiaomin,Ren, Shouwen,Fu, Yanfeng,Li, Lan,Li, Lan.

[9]Cloning, sequencing and identification of single nucleotide polymorphisms of partial sequence on the porcine CACNA1S gene. Fang XiaoMin,Ren ShouWen,Fang XiaoMin,Xu NingYing. 2008

[10]Characterization of the porcine p65 subunit of NF-kappa B and its association with virus antibody levels. Cao, Jiyue,Li, Hegang,Ma, Guojian,Gui, Duan,Zhao, Shuanping,Wang, Pan,Ruan, Jinxue,Yang, Shulin,Li, Kui,He, Kongwang,Wang, Xuemin. 2011

[11]Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae. Sun, Zhiyuan,Liu, Maojun,Zou, Huafeng,Li, Xian,Zhao, Ruqian,Liu, Maojun,Shao, Guoqing. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台